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| Status |
Public on May 13, 2021 |
| Title |
Dynamic imaging of nascent RNA reveals general principles of transcription dynamics and pervasive recursive splicing [primer extension] |
| Organism |
Homo sapiens |
| Experiment type |
Other
|
| Summary |
The coupled activities of RNA polymerase and the spliceosome are responsible for the abundance and isoform composition of mRNA in human cells. However, the dynamics of these megadalton enzymatic complexes working in concert on endogenous genes have not been described. Here, we establish a quasi-genome-scale platform for observing synthesis and processing kinetics of single nascent RNA molecules in real time. We find that all observed genes show transcriptional bursting. We also observe large kinetic variation in intron removal for single introns in single cells which is inconsistent with deterministic splice site selection. Transcriptome-wide footprinting of the U2AF complex further reveals widespread splice site selection within introns. We propose and validate a unified theoretical model for transcription and splicing based on pervasive stochastic recursive splicing.
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| |
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| Overall design |
splicing intermediates detection by primer extension sequencing
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| |
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| Contributor(s) |
Wan Y, Anastasakis DG, Hafner M, Larson DR |
| Citation(s) |
33979654 |
| |
| Submission date |
Jul 17, 2019 |
| Last update date |
May 13, 2021 |
| Contact name |
Daniel R Larson |
| E-mail(s) |
yihan.wan@nih.gov
|
| Organization name |
NIH
|
| Street address |
41 Medlar Dr.
|
| City |
Bethesda |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (1) |
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| This SubSeries is part of SuperSeries: |
| GSE134377 |
Dynamic imaging of nascent RNA reveals general principles of transcription dynamics and pervasive recursive splicing |
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| Relations |
| BioProject |
PRJNA555416 |
| SRA |
SRP215462 |
