|
| Status |
Public on Aug 14, 2019 |
| Title |
DNA methylation analysis of HCT116 after knockdown and rescue of UHRF1 |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by genome tiling array
|
| Summary |
In HCT116 colorectal cancer cells, UHRF1 was knocked down by shRNA (puromycin) while simultaneously transduced with wildtype or mutant UHRF1 (blasticidin) or NDI1 (- control) followed by dual antibiotic selection. DNA was analyzed 11 days after viral transduction.
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| |
|
| Overall design |
Bisulphite converted DNA from the 4 samples were hybridised to the Illumina Infinium MethylationEPIC BeadChip.
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| |
|
| Contributor(s) |
Vaughan R, Rothbart S, Dickson B |
| Citation(s) |
31481468 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R35 GM124736 |
Molecular mechanisms of chromatin and epigenetic regulation |
VAN ANDEL RESEARCH INSTITUTE |
Scott Rothbart |
|
| |
| Submission date |
Aug 13, 2019 |
| Last update date |
Mar 24, 2020 |
| Contact name |
Scott Rothbart |
| E-mail(s) |
scott.rothbart@vai.org
|
| Organization name |
Van Andel Research Institute
|
| Department |
Center for Epigenetics
|
| Lab |
Rothbart Lab
|
| Street address |
333 Bostwick Ave NE
|
| City |
Grand Rapids |
| State/province |
MI |
| ZIP/Postal code |
49503 |
| Country |
USA |
| |
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| Platforms (1) |
|
| Samples (4)
|
|
| Relations |
| BioProject |
PRJNA560094 |
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