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| Status |
Public on Aug 20, 2019 |
| Title |
Gene expression of E.coli challenged green rice leafhopper Nephotettix cincticeps. |
| Organism |
Nephotettix cincticeps |
| Experiment type |
Expression profiling by array
|
| Summary |
The green rice leafhopper Nephotettix cincticeps have two mutualistic symbiotic bacteria (Candidatus Sulcia muelleri and Candidatus Nasuia deltocephalinicola) in its symbiont special organ bacteriome and are also infected to rickettsia. In order to determine immune challenge is induced or not in N. cincticeps, we investigated gene expression of Escherichia coli challenged N. cincticeps.
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| Overall design |
One day adult N. cincticeps females were pricked with E. coli with a sterile needle. Control adults were pricked with sterilized water. Total RNA of five females was extracted after 3 h and 12 h incubation respectively. E.coli challenged and control Nephotettix cincticeps samples were prepared 3 replicates for 3h and 4 replicates for 12h.
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| Contributor(s) |
Tomizawa M, Nakamura Y, Noda H |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Aug 19, 2019 |
| Last update date |
Aug 23, 2019 |
| Contact name |
Yuki Nakamura |
| E-mail(s) |
ykn901014@gmail.com
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| Organization name |
National Institute of Agrobiololgical Sciences
|
| Street address |
1-1 Owashi
|
| City |
Tsukuba |
| State/province |
Ibaraki |
| ZIP/Postal code |
305-8634 |
| Country |
Japan |
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| Platforms (1) |
| GPL27108 |
Agilent-027323 Nephotettix cincticeps NC2010A |
|
| Samples (14)
|
| GSM4038749 |
Sample 1: Control cy3 vs E. coli challenged cy5 for 3h |
| GSM4038750 |
Sample 2: Control cy5 vs E. coli challenged cy3 for 3h |
| GSM4038751 |
Sample 3: Control cy3 vs E. coli challenged cy5 for 3h |
| GSM4038752 |
Sample 4: Control cy5 vs E. coli challenged cy3 for 3h |
| GSM4038753 |
Sample 5: Control cy3 vs E. coli challenged cy5 for 3h |
| GSM4038754 |
Sample 6: Control cy5 vs E. coli challenged cy3 for 3h |
| GSM4038755 |
Sample 7: Control cy3 vs E. coli challenged cy5 for 12h |
| GSM4038756 |
Sample 8: Control cy5 vs E. coli challenged cy3 for 12h |
| GSM4038757 |
Sample 9: Control cy3 vs E. coli challenged cy5 for 12h |
| GSM4038758 |
Sample 10: Control cy5 vs E. coli challenged cy3 for 12h |
| GSM4038759 |
Sample 11: Control cy3 vs E. coli challenged cy5 for 12h |
| GSM4038760 |
Sample 12: Control cy5 vs E. coli challenged cy3 for 12h |
| GSM4038761 |
Sample 13: Control cy3 vs E. coli challenged cy5 for 12h |
| GSM4038762 |
Sample 14: Control cy5 vs E. coli challenged cy3 for 12h |
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| Relations |
| BioProject |
PRJNA560866 |
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