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| Status |
Public on Sep 14, 2020 |
| Title |
Pervasive promoter-proximal regulation in archaea: Permanganate ChIP-seq hydrogen-peroxide treated cells |
| Organism |
Saccharolobus solfataricus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Recruitment of RNA polymerase and initiation factors to the promoter is the only known mechanisms for transcription activation and repression in archaea. Whether any of the subsequent steps towards productive transcription elongation is involved in regulation is not known. We characterised how the basal transcription machinery is distributed along genes in the archaeon Sulfolobus solfataricus. We discovered a distinct early elongation phase where RNA polymerases sequentially recruit the elongation factors Spt4/5 and Elf1 to form the transcription elongation complex (TEC) before the TEC escapes into productive transcription. TEC escape is rate-limiting for transcription output during exponential growth. Oxidative stress causes changes in TEC escape that correlate with changes in the transcriptome. Our results thus establish that TEC escape contributes to the basal promoter strength and facilitates transcription regulation. Impaired TEC escape coincides with the accumulation of initiation factors at the promoter and recruitment of termination factor aCPSF1 to the early TEC. This suggests two possible mechanisms for how TEC escape limits transcription, physically blocking upstream RNA polymerases during transcription initiation and premature termination of early TECs.
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| |
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| Overall design |
Permanganate ChIP-seq with polyclonal antibodies
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| |
|
| Contributor(s) |
Werner F, Blombach F |
| Citation(s) |
38388540 |
| |
| Submission date |
Dec 04, 2019 |
| Last update date |
Mar 07, 2024 |
| Contact name |
Finn Werner |
| Organization name |
University College London
|
| Department |
Institute of Structural and Molecular Biology
|
| Street address |
Gower Street
|
| City |
London |
| ZIP/Postal code |
WC1E 6BT |
| Country |
United Kingdom |
| |
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| Platforms (1) |
| GPL27815 |
Illumina HiSeq 2500 (Saccharolobus solfataricus) |
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| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE141290 |
Pervasive promoter-proximal regulation in archaea |
|
| Relations |
| BioProject |
PRJNA593549 |
| SRA |
SRP234717 |
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