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| Status |
Public on Mar 30, 2020 |
| Title |
Release of promoter-proximal paused Pol II in response to histone deactylatase inhibition |
| Organism |
Drosophila melanogaster |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
Expression profiling by high throughput sequencing
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| Summary |
A correlation between histone acetylation and transcription has been noted for a long time, but little is known about what step(s) in the transcription cycle is influenced by acetylation. Here, we have examined the immediate transcriptional response to histone deacetylase (HDAC) inhibition, and find that release of promoter-proximal paused RNA polymerase II (Pol II) into elongation is stimulated, whereas recruitment to gene promoters is not. Although histone acetylation is elevated globally by HDAC inhibition, less than 100 genes respond within 10 min. These genes are highly paused, are strongly associated with the chromatin regulators NURF and Trithorax, display a greater increase in acetylation of the first nucleosomes than other genes, and become transcriptionally activated by HDAC inhibition. Among these rapidly upregulated genes are HDAC1 (Rpd3) and subunits of HDAC-containing co-repressor complexes, demonstrating feedback regulation upon HDAC inhibition. Our results suggest that histone acetylation stimulates transcription of paused genes, and that increased acetylation is not a consequence of their enhanced expression.
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| Overall design |
Examination of nascent RNA (PRO-seq), 2 different histone modifications (ChIP-seq) and accessible chromatin (ATAC-seq) from Drosophila S2 cells treated with HDAC inhibitor (TSA) for 10 min or 30 min along with DMSO as control drug.
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| |
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| Contributor(s) |
Vaid R, Wen J, Mannervik M |
| Citation(s) |
32297950 |
| |
| Submission date |
Dec 11, 2019 |
| Last update date |
May 26, 2020 |
| Contact name |
Jiayu Wen |
| Organization name |
John Curtin School of Medical Research, The Australian National University
|
| Department |
Department of Genome Science
|
| Lab |
The Wen Lab
|
| Street address |
133 Garran Rd
|
| City |
CANBERRA |
| State/province |
ACT |
| ZIP/Postal code |
2601 |
| Country |
Australia |
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| Platforms (1) |
| GPL22106 |
NextSeq 550 (Drosophila melanogaster) |
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| Samples (30)
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| Relations |
| BioProject |
PRJNA594989 |
| SRA |
SRP237262 |
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