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| Status |
Public on Feb 19, 2021 |
| Title |
Transcriptome analysis of Rhodobacter sphaeroides 2.4.1 wildtype and the two overexpressing strains OE_RSP_6037 and OE_CcsR |
| Organism |
Cereibacter sphaeroides |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Many protein domains are conserved among different classes and are found in numerous species, but their function remains obscure. Data bases list more than 17,000 DUF1127 proteins but to date no biological function could be assigned to any of these proteins. This study demonstrates that the 71 amino acid DUF1127 protein CcaF1 from the alphaproteobacterium Rhodobacter sphaeroides participates in the maturation of the CcsR sRNAs that are processed from the 3´ UTR of the ccaF mRNA and have a role in the oxidative stress defense. CcaF1 also affects the abundance of several other sRNAs but also of tRNAs, rRNAs and several mRNAs. Co-immunoprecipitation revealed direct binding of CcaF1 to many cellular RNAs of different type and an effect of CcaF1 on the stability of selected RNAs was demonstrated. Thus, the widely distributed DUF1127 domain can mediate RNA-binding and affect stability of its binding partners and consequently the bacterial transcriptome.
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| Overall design |
This data includes the transcriptome data of three different exponentially growing strains each in triplicates: Rhodobacter sphaeroides 2.4.1 (wildtype), R. sphaeroides 2.4.1 pRK4352_RSP_6037 (constitutively overexpression of RSP_6037) and R. sphaeroides 2.4.1 pRK4352_CcsR1-4 (constitutively overexpression of the sRNAs CcsR1-4). Total RNA of all strains was isolated and sequenced.
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| Contributor(s) |
Grützner J, Spanka D, Monzon VA, Klug G |
| Citation(s) |
33706375 |
| |
| Submission date |
Jan 30, 2020 |
| Last update date |
Apr 20, 2021 |
| Contact name |
Gabriele Klug |
| E-mail(s) |
Gabriele.Klug@mikro.bio.uni-giessen.de
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| Organization name |
Justus-Liebig University Gießen
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| Department |
Molecular- and Microbiology
|
| Street address |
Heinrich-Buff-Ring 26-32
|
| City |
Gießen |
| ZIP/Postal code |
35392 |
| Country |
Germany |
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| Platforms (1) |
| GPL24048 |
Illumina NextSeq 500 (Rhodobacter sphaeroides) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA603915 |
| SRA |
SRP246085 |
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