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| Status |
Public on May 19, 2020 |
| Title |
Drosophila SWR1 and NuA4 complexes originate from DOMINO splice isoforms [RNA-seq] |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Histone acetylation and deposition of H2A.Z variant are integral aspects of active transcription. In Drosophila, the single DOMINO chromatin regulator complex is thought to combine both activities via an unknown mechanism. Here we show that two alternative splice isoforms of the DOMINO nucleosome remodeling ATPase, DOM-A and DOM-B, directly specify two distinct multi-subunit complexes. Both complexes are necessary for transcriptional regulation but through different mechanisms. The DOM-B complex incorporates H2A.V (the fly ortholog of H2A.Z) genome-wide in an ATP-dependent manner, like the yeast SWR1 complex. The DOM-A complex, instead, functions as an ATP-independent histone acetyltransferase complex similar to the yeast NuA4, targeting lysine 12 of histone H4. Our work provides an instructive example of how different evolutionary strategies lead to similar functional separation.
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| |
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| Overall design |
RNAseq experiment of control (GST/GFP), H2A.v, Domino-A, Domino-B and Tip60 RNAi in 3 biological replicates.
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| |
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| Contributor(s) |
Scacchetti A, Schauer T, Becker PB |
| Citation(s) |
32432549 |
| |
| Submission date |
Feb 13, 2020 |
| Last update date |
May 26, 2020 |
| Contact name |
Tamas Schauer |
| E-mail(s) |
tamas.schauer@helmholtz-munich.de
|
| Organization name |
Helmholtz Zentrum München
|
| Department |
Institute of Epigenetics and Stem Cells
|
| Street address |
Feodor-Lynen-Straße 21
|
| City |
Munich |
| ZIP/Postal code |
81377 |
| Country |
Germany |
| |
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| Platforms (1) |
| GPL19951 |
Illumina HiSeq 1500 (Drosophila melanogaster) |
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| Samples (15)
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| This SubSeries is part of SuperSeries: |
| GSE145738 |
Drosophila SWR1 and NuA4 complexes originate from DOMINO splice isoforms |
|
| Relations |
| BioProject |
PRJNA606578 |
| SRA |
SRP249359 |
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