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| Status |
Public on Mar 16, 2020 |
| Title |
Different Egyptian rousette interferon omega proteins elicit an overlapping but not identical transcriptional response in bat cells. |
| Organism |
Rousettus aegyptiacus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Abstract from accompanying publication: "Bats host a number of viruses that cause severe disease in humans without experiencing overt symptoms of disease themselves. While the mechanisms underlying this ability to avoid sickness are not known, deep sequencing studies of bat genomes have uncovered genetic adaptations that may have functional importance in the antiviral response of these animals. Egyptian rousette bats (Rousettus aegyptiacus) are the natural reservoir hosts of Marburg virus (MARV). In contrast to humans, these bats do not become sick when infected with MARV. A striking difference to the human genome is that Egyptian rousettes have an expanded repertoire of IFNW genes. To probe the biological implications of this expansion, we synthesized IFN-ω4 and IFN-ω9 proteins and tested their antiviral activity in Egyptian rousette cells. Both IFN-ω4 and IFN-ω9 showed antiviral activity against RNA viruses, including MARV, with IFN-ω9 being more efficient than IFN-ω4. Using RNA-Seq, we examined the transcriptional response induced by each protein. Although the sets of genes induced by the two IFNs were largely overlapping, IFN-ω9 induced a more rapid and intense response than did IFN-ω4. About 13% of genes induced by IFN-ω treatment are not found in the Interferome or other ISG databases, indicating that they may be uniquely IFN-responsive in this bat."
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| Overall design |
mRNA trancriptional profiles of bat cells pretreated with bat interferons, media, universal interferon, or an unrelated protein for 4 or 8 hours at three different concentrations, in triplicate, sequenced on Illumina HiSeq 2500
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| Contributor(s) |
Pavlovich SS, Hume AJ, Feng F, Mühlberger E, Kepler TB |
| Citation(s) |
32231668 |
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| Submission date |
Feb 23, 2020 |
| Last update date |
Apr 06, 2020 |
| Contact name |
Stephanie Sarah Pavlovich |
| E-mail(s) |
sdsouza@bu.edu
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| Organization name |
Boston University School of Medicine
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| Department |
Microbiology
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| Lab |
Kepler Lab
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| Street address |
700 Albany St, W508D
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| City |
Boston |
| State/province |
Massachusetts |
| ZIP/Postal code |
02118 |
| Country |
USA |
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| Platforms (1) |
| GPL24487 |
Illumina HiSeq 2500 (Rousettus aegyptiacus) |
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| Samples (66)
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| Relations |
| BioProject |
PRJNA608171 |
| SRA |
SRP250411 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE145761_RAW.tar |
5.6 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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