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| Status |
Public on Feb 28, 2020 |
| Title |
U1A iCLIP-seq (individual-nucleotide resolution UV crosslinking and immunoprecipitation coupled with RNA sequencing) analysis in HeLa cells |
| Organism |
Homo sapiens |
| Experiment type |
Other
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| Summary |
To better understand the mechanism of U1A functions in human cells, we performed U1A iCLIP-seq analysis in HeLa cells. iCLIP-seq is a previously well-established protocol that is believed to detect protein-RNA interaction at individual-nucleotide resolution. Indeed, successful completion of U1A iCLIP-seq has helped us to illuminate more detailed mechanism through which U1 snRNP prevents mRNA PCPA (premature cleavage and polyadenylation)
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| Overall design |
HeLa cells were grown in 10 cm cell dishes, protein-RNA were crosslinked by UV light exposure. U1A-RNA were immunoprecipitated by anti-U1A antibody (santa cruz : sc-101149). Immunoprecipitated RNAs were further purified and prepared for RNA-seq library construction. For detailed protocol, please check the published protocol (Methods. 2014 Feb; 65(3): 274–287.)
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| Contributor(s) |
Deng Y, Shi J, Ran Y, Xiang AP, Yao C |
| Citation(s) |
32828285 |
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| Submission date |
Feb 27, 2020 |
| Last update date |
Aug 30, 2020 |
| Contact name |
chengguo yao |
| E-mail(s) |
yaochguo@mail.sysu.edu.cn
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| Organization name |
sysu
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| Department |
school of medicine
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| Street address |
No. 74 zhongshan 2rd road
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| City |
guangzhou |
| ZIP/Postal code |
510080 |
| Country |
China |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA609061 |
| SRA |
SRP250922 |
