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| Status |
Public on Apr 28, 2020 |
| Title |
Spatiotemporal single-cell RNA sequencing of developing chicken hearts identifies interplay between cellular differentiation and morphogenesis |
| Organism |
Gallus gallus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Single-cell RNA sequencing is a powerful tool to study developmental biology but does not preserve spatial information about tissue morphology and cellular interactions. Here, we combine single-cell and spatial transcriptomics with algorithms for data integration to study the development of the chicken heart from the early to late four-chambered heart stage. We create a census of the diverse cellular lineages in developing hearts, their spatial organization, and their interactions during development. Spatial mapping of differentiation transitions in cardiac lineages defines transcriptional differences between epithelial and mesenchymal cells within the epicardial lineage. Using spatially resolved expression analysis, we identify anatomically restricted expression programs, including expression of genes implicated in congenital heart disease. Last, we discover a persistent enrichment of the small, secreted peptide, thymosin beta-4, throughout coronary vascular development. Overall, our study identifies an intricate interplay between cellular differentiation and morphogenesis.
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| Overall design |
Spatially-resolved RNA-seq with high-throughput time-course scRNA-seq to study the gene expression, interactions, and regulatory programs that drive fetal development of the chicken hearts.
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| |
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| Contributor(s) |
Mantri M, Scuderi GJ, Nassab RA, Butcher JT, De Vlaminck I |
| Citation(s) |
33741943, 33846360 |
| |
| Submission date |
Apr 27, 2020 |
| Last update date |
May 06, 2021 |
| Contact name |
Iwijn De Vlaminck |
| E-mail(s) |
id93@cornell.edu
|
| Organization name |
Cornell University
|
| Department |
Meinig School of Biomedical Engineering
|
| Lab |
De Vlaminck Lab
|
| Street address |
Weill Hall, 237 Tower Road
|
| City |
Ithaca |
| State/province |
New York |
| ZIP/Postal code |
14853 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL19787 |
Illumina NextSeq 500 (Gallus gallus) |
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| Samples (11)
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| Relations |
| BioProject |
PRJNA628708 |
| SRA |
SRP258747 |
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