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| Status |
Public on Jul 23, 2020 |
| Title |
Effect of cpc-1 deletion in Neurospora crassa on transcriptome under different culture conditions |
| Organism |
Neurospora crassa |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The high throughput mRNA-seq technology was used to measure the effect of cpc-1 deletion on transcriptome change of Neurospora crassa grown under different culture conditions using standard Illumina protocols.
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| Overall design |
The transcription profiles of wild-type (WT) strain FGSC4200 and cpc-1 deletion mutant (cpc-1∆) of Neurospora crassa were generated by deep sequencing using Illumina sequencing platform. The WT and cpc-1∆ strains were cultured in 2% glucose medium (1 × Vogel’s, 2% glucose), 0.1% glucose medium (1 × Vogel’s, 0.1% glucose and 0.17% arginine) or 2% glucose medium with 5 mM 3-AT. A 5’ epitope-tagged versions of cpc-1(WT) ORF under the control of the ccg-1 promoter and the ccg-1 5’ untranslated region (5’ UTR) was expressed in cpc-1∆ strain. This strain (cpc-1KO+cpc-1(WT)) was cultured in 2% glucose medium with 5 mM 3-AT.
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| Contributor(s) |
Lyu X, Liu Y |
| Citation(s) |
33051373 |
| |
| Submission date |
May 11, 2020 |
| Last update date |
Oct 22, 2020 |
| Contact name |
xueliang lyu |
| E-mail(s) |
lvxueliang0715@aliyun.com
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| Organization name |
UT Southwestern Medical Center
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| Department |
Physiology
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| Lab |
Yi Liu
|
| Street address |
6001 Forest Park Road
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| City |
Dallas |
| State/province |
Texas |
| ZIP/Postal code |
75235 |
| Country |
USA |
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| Platforms (2) |
| GPL16164 |
Illumina HiSeq 2000 (Neurospora crassa) |
| GPL26551 |
Illumina NovaSeq 6000 (Neurospora crassa) |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA631721 |
| SRA |
SRP261117 |
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