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| Status |
Public on May 23, 2020 |
| Title |
Transcriptional and functional analysis of CD1c+ human dendritic cells identifies a CD163+ subset priming CD8+CD103+ T cells [Breast_iLN] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Dendritic cells (DC) are antigen presenting cells controlling T cell activation. In human, the diversity, ontogeny and functional capabilities of DC subsets are not fully understood. Here, we identified circulating CD88-CD1c+CD163+ DC (termed as DC3) as an immediate precursor of inflammatory CD88-CD14+CD1c+CD163+FcεRI+ DC. DC3 develop via a specific pathway activated by GM-CSF, independent from the cDC-restricted (CDP) and monocyte-restricted (cMoP) progenitors. As classical DC, but unlike monocytes, DC3 drove the activation of naïve T cells. In vitro, DC3 displayed a distinctive ability to prime CD8+ T cells expressing a tissue-homing signature and the epithelial homing alpha-E integrin (CD103) through transforming growth factor-b (TGF-β) signaling. In vivo, DC3 infiltrated luminal breast cancer primary tumors and DC3 infiltration correlated positively with CD8+CD103+CD69+ tissue-resident memory T cells. Altogether, these findings define DC3 as a lineage of inflammatory DC endowed with a strong potential to regulate tumor immunity.
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| Overall design |
Tumor-draining lymph nodes (tdLN) and primary tumors were collected and processed within few hours after the primary surgery. CD11c+HLADR+CD14-CD1a-CD206-CD1c+, CD11c+HLADR+CD1a-CD1c-CD14+ and CD11c+HLADR+CD1a-CD1c+CD14+ cells were sorted in triplicates with up to 1000 cells per sample.
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| Contributor(s) |
Bourdely P, Vaivode K, Anselmi G, Ramos RN, Missolo-Kousou Y, Hidalgo S, Tosselo J, Nunez N, Richer W, Vincent-Salomon A, Saxena A, Wood K, Lladser A, Piaggio E, Helft J, Guermonprez P |
| Citation(s) |
32610077 |
| |
| Submission date |
May 22, 2020 |
| Last update date |
Aug 24, 2020 |
| Contact name |
Kristine Vaivode |
| E-mail(s) |
kristine.vaivode@kcl.ac.uk
|
| Phone |
02078486907
|
| Organization name |
King's College London
|
| Department |
Centre for Inflammation Biology and Cancer Immunology
|
| Lab |
Dr Pierre Guermonprez lab
|
| Street address |
CIBCI, First Floor, New Hunt's House, Great Maze Pond
|
| City |
London |
| ZIP/Postal code |
SE1 1UL |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (9)
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| This SubSeries is part of SuperSeries: |
| GSE151095 |
Transcriptional and functional analysis of CD1c+ human dendritic cells identifies a CD163+ subset priming CD8+CD103+ T cells |
|
| Relations |
| BioProject |
PRJNA634547 |
| SRA |
SRP263000 |
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