|
| Status |
Public on Jul 15, 2020 |
| Title |
ChIP-seq analysis of SNAIL transcription factor binding sites in RH30 rhabdomyosarcoma cells |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
ChIP-seq analysis of SNAIL binding sites in RH30 cells was performed to discover novel SNAIL binding sites in rhabdmyosarcoma cells.
|
| |
|
| Overall design |
ChIP was performed using SimpleChip Enzymatic Chromatin IP Kit (Cell Signaling Technology) according to the manufacturer’s protocol. For ChIP assays 10 µg of goat antibody against SNAIL (R&D Systems, cat. AB-108-C), . After immunoprecipitation, the DNA was isolated using spin columns from the kit. Subsequently, ChIP-Seq analysis of the immunoprecipitated sample and input sample was performed using the Intelliseq sp. z o. o. (Cracow, Poland) company service in cooperation with Novogene Co., Lt d (Cambridge, UK) using Illumina high-throughput sequencer.
|
| |
|
| Contributor(s) |
Skrzypek K, Majka M |
| Citation(s) |
32664538, 34094709 |
| |
| Submission date |
Jun 12, 2020 |
| Last update date |
Jun 08, 2021 |
| Contact name |
Klaudia Skrzypek |
| E-mail(s) |
klaudia.skrzypek@uj.edu.pl
|
| Phone |
+48126591593
|
| Organization name |
Jagiellonian University Medical College
|
| Department |
Department of Transplantation
|
| Street address |
Wielicka 265
|
| City |
Krakow |
| ZIP/Postal code |
30-663 |
| Country |
Poland |
| |
|
| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
|
| Samples (2) |
|
| Relations |
| BioProject |
PRJNA639097 |
| SRA |
SRP267071 |
