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| Status |
Public on Aug 30, 2022 |
| Title |
Effect of microRNA-27b-3p mimic treatment on human osteoarthritis fibroblast-like synoviocyte gene expression profiles |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
OBJECTIVE: To identify the changes in gene expression elicited by miR-27b-3p mimic transfection of human osteoarthritis (OA) primary fibroblast-like synoviocytes (FLS) obtained from patients undergoing knee arthroplasty. Cells were cultured, and treated for 48 h with miR-27b-3p mimic or Cel-miR-39-3p control mimic. Total RNA was then extracted, and mRNA libraries were prepared using Illumina's TruSEQ stranded total RNA library preparation kit, pooled together, and sequenced on Illumina's NextSeq550.
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| Overall design |
Differential expression analysis was performed on the RNA-SEQ data obtained from 6 prepared libraries, where 3 were treated with the miR-27b-3p mimic and 3 with Cel-miR-39-3p control mimic. Subsequent pathway analysis identified the upregulation of ECM-related genes and pathways suggesting that miR-27b-3p is involved in the modulation of synovial ECM production.
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| Contributor(s) |
Tavallaee G, Lively S, Ali SA, Im M, Sarda C, Rossomacha E, Nakamura S, Potla P, Shestopaloff K, Matelski J, Ratneswaran A, Perry K, Mitchell GM, Ghandhi R, Rockel JS, Hinz B, Jurisica I, Kapoor M |
| Citation(s) |
35791923 |
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| Submission date |
Jun 16, 2020 |
| Last update date |
Nov 29, 2022 |
| Contact name |
Mohit Kapoor |
| E-mail(s) |
mohit.kapoor@uhnresearch.ca
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| Organization name |
Schroeder Arthritis Institute
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| Street address |
60 Leonard Ave
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| City |
Toronto |
| State/province |
Ontario |
| ZIP/Postal code |
M5T 0S8 |
| Country |
Canada |
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| Platforms (1) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA639874 |
| SRA |
SRP267587 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE152638_GTavallaee_TotalRNAseq_genecounts.tsv.gz |
713.3 Kb |
(ftp)(http) |
TSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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