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| Status |
Public on Apr 30, 2021 |
| Title |
Comprehensive single cell RNA-seq datasets of mice trachea epithelial progenitors in 6 time points from E12.5 to E18.5 and mature epithelial cells in 2-month mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We delineate the comprehensive developmental trajectories of trachea epithelial progenitors in mice using scRNA-seq datasets. After obtaining the scRNA-seq data in 6 time points from E12.5 to E18.5, we merged all the datasets to describe the establishment process of basal cell population during development, which works as airway facultative stem cells. We found that Krt17 is a novel intermediate marker for basal cell progenitors and Id genes are key transcriptional factors for balancing the proliferation and differentiation programs by promoting their cell cycle.
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| Overall design |
Transcriptomic analyses at single cell resolution of tracheal epithelial cells in 7 time points (E12.5, E13.5, E14.5, E15.5, E16.5, E18.5, and 2 months)
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| Contributor(s) |
Kiyokawa H, Morimoto M |
| Citation(s) |
34129836 |
| |
| Submission date |
Jun 17, 2020 |
| Last update date |
Jul 30, 2021 |
| Contact name |
Hirofumi Kiyokawa |
| E-mail(s) |
h.kiyokawa@gmail.com
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| Phone |
81783063199
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| Organization name |
Riken
|
| Department |
Center for Biosystems Dynamics Research
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| Lab |
Laboratory for lung development and regenration
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| Street address |
2-2-3 Minatojima-minamimachi, Chuo-ku
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| City |
Kobe |
| State/province |
Hyogo |
| ZIP/Postal code |
6500047 |
| Country |
Japan |
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| Platforms (1) |
| GPL18480 |
Illumina HiSeq 1500 (Mus musculus) |
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| Samples (7)
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| Relations |
| BioProject |
PRJNA640145 |
| SRA |
SRP267715 |
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