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| Status |
Public on Aug 03, 2021 |
| Title |
Systemic persistent RNA virus infection is short-lived at the single cell level |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We report that cells infected with the prototypic arenavirus LCMV are continiously cleared from the infection in a non-cytolytic manner. Non-cytolytic clearance of murine hepatocytes goes along with profound alterations in gene expression.
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| Overall design |
We sorted hepatocytes from 14 week-old Cre-reporting mice (STOP_flox_RFP), which had been neonatally infected with Cre-encoding LCMV according to RFP expression (RFP-positive and RFP-negative). Total cellular RNA of each population was subject to bulk RNAseq. RFP-negative hepatocytes from naive STOP_flox_RFP mice and RFP-positive hepatocytes from naive mice expressing RFP under a liver-specific promoter (STOP_flox_RFP x Alb-Cre) served as controls
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| Contributor(s) |
Reuther P, Martin K, Geier F, Pinschewer DD |
| Citation(s) |
34398180 |
| |
| Submission date |
Sep 03, 2020 |
| Last update date |
Nov 18, 2021 |
| Contact name |
DBM Bioinformatics Core Facility |
| Phone |
+41612073541
|
| Organization name |
University of Basel
|
| Department |
Departement of Biomedicine
|
| Street address |
Hebelstrasse 20
|
| City |
Basel |
| State/province |
BS |
| ZIP/Postal code |
4053 |
| Country |
Switzerland |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (19)
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| Relations |
| BioProject |
PRJNA661293 |
| SRA |
SRP279985 |
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