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| Status |
Public on Apr 24, 2009 |
| Title |
Homogeneous monocytes and macrophages from hES cells following coculture-free differentiation in M-CSF and IL-3. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
In the present study we aimed at producing homogeneous populations of monocytes and macrophages from human embryonic stem (hES) cells. Human embryonic stem cell lines KCL001, KCL002, and HUES-2 were differentiated into monocytes by coculture-free differentiation with two growth factors using a three-step method. The method involved embryoid body (EB) formation in hES media, directed differentiation with macrophage colony-stimulating factor and interleukin (IL)-3, and harvest of nonadherent monocytes from the culture supernatants. Transcriptome analysis of the esMC and the esMDM revealed a distinct myeloid signature that correlated with primary adult blood-derived monocytes and spleen tissue samples but not with other tissue samples tested. In conclusion we have developed a simple and efficient method for producing homogeneous populations of monocytes and macrophages from hES cells. esMCs have a myeloid signature and can differentiate into functional macrophages. The method should prove useful in answering experimental questions regarding monocyte and macrophage development and biology.
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| Overall design |
Triplicates of monocytes and macrophages derived from human embryonic stem cells were compared to a triplicate of freshly isolated human blood monocytes.
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| Contributor(s) |
Karlsson K, Cowley S, Martinez Estrada FO, James W |
| Citation(s) |
18550257 |
| |
| Submission date |
Apr 23, 2009 |
| Last update date |
Jul 26, 2018 |
| Contact name |
Fernando O Martinez Estrada |
| E-mail(s) |
f.martinezestrada@surrey.ac.uk
|
| Organization name |
University of Surrey
|
| Department |
Faculty of Health & Medical Sciences
|
| Lab |
Innate Immunity
|
| Street address |
Stag Hill
|
| City |
Guildford |
| State/province |
Surrey |
| ZIP/Postal code |
GU2 7XH |
| Country |
United Kingdom |
| |
|
| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (9)
|
| GSM396480 |
Human embryonic cell-derived monocytes 1 |
| GSM396481 |
Human embryonic cell-derived monocytes 2 |
| GSM396482 |
Human embryonic cell-derived monocytes 3 |
|
| Relations |
| BioProject |
PRJNA116873 |
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