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| Status |
Public on Dec 21, 2020 |
| Title |
Gene expression profiling using RNA-Seq data of MMTV-Wnt1 tumor tissue from mice treated with E7386 |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We conducted an RNA-Seq analysis using total RNA extracted from MMTV-Wnt1 tumor tissues resected from mice orally administered E7386 at 25 or 50 mg/kg twice daily, or vehicle, for 3 days (Day 4) or 7 days (Day 8) and examined the differentially expressed gene levels compared with vehicle at days 4 and 8.
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| Overall design |
MMTV-Wnt1 transgenic mice (B6SJL-Tg(Wnt1)1Hev/J) were obtained from the Jackson Laboratory (Bar Harbor, ME). Spontaneously developed breast tumors were resected from the MMTV-Wnt1 transgenic mice, diced, and inoculated into C57BL/6J mice (Charles River Laboratories Japan). Tumors were allowed to grow to approximately 200 mm3 in size (designated Day 1); tumor-bearing mice were then randomly allocated to treatment groups and orally administered E7386 (25.0, or 50.0 mg/kg twice daily) for 3 days (Day 4) or 7 days (Day 8).
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| Contributor(s) |
Yamada K, Hori Y, Kimura T, Funahashi Y, Ozawa Y |
| Citation(s) |
33408116 |
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| Submission date |
Sep 29, 2020 |
| Last update date |
May 25, 2022 |
| Contact name |
Kazuhiko Yamada |
| E-mail(s) |
k7-yamada@hhc.eisai.co.jp
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| Organization name |
Eisai Co., Ltd.
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| Street address |
5-1-3 Tokodai
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| City |
Tsukuba-shi |
| ZIP/Postal code |
300-2635 |
| Country |
Japan |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA666332 |
| SRA |
SRP285759 |
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