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| Status |
Public on Mar 06, 2021 |
| Title |
Yeast cell fate control by temporal redundancy modulation of transcription factor paralogs |
| Organisms |
Saccharomyces cerevisiae; Nakaseomyces glabratus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Recent single-cell studies have revealed that yeast stress response involves multiple transcription factors that are temporally activated in pulses. However, it remains largely unclear whether and how these dynamic transcription factors temporally interact to regulate stress survival. Here we show that budding yeast cells can exploit the temporal relationship between paralogous general stress regulators, Msn2 and Msn4, during stress response. We found that individual pulses of Msn2 and Msn4 are largely redundant, and cells can enhance the expression of their shared target genes by increasing their temporal divergence. Thus, functional redundancy between these two paralogs is modulated in a dynamic manner to confer fitness advantages for yeast cells, which might feed back to promote the preservation of their functional redundancy. This evolutionary implication was supported by evidence from Msn2/Msn4 orthologs and analyses of other transcription factor paralogs. Together, we show a cell fate control mechanism through temporal redundancy modulation in yeast, which may represent an evolutionarily important strategy for maintaining functional redundancy between gene duplicates.
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| Overall design |
First, for transient stimulation experiments, 7 genotypes (namely, 3 genotypes of S. cerevisiae: wild type sc, Msn2_sc deletion, Msn4_sc deletion and 4 genotypes of C. glabrata: wild type cg, Msn2_cg deletion, Msn4_cg deletion, Msn2_cg deletion Msn4_cg deletion) under two conditions (namely, stressed and control) were sequenced with 2 biological repeats. Second, for steady-state experiments, wild type sc strain was sequenced under 4 different carbon stresses with 2 biological repeats.
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| Contributor(s) |
Wu Y, Wu J, Deng M, Lin Y |
| Citation(s) |
34035307 |
| BioProject |
PRJNA675209 |
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| Submission date |
Nov 12, 2020 |
| Last update date |
May 27, 2021 |
| Contact name |
Yan WU |
| E-mail(s) |
wy-fcb@pku.edu.cn
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| Organization name |
Peking University
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| Department |
Academy for Advanced Interdisciplinary Studies
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| Lab |
Yihan Lin
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| Street address |
No.5 Yiheyuan Road Haidian District, Beijing, P.R.China
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| City |
Beijing |
| State/province |
Beijing |
| ZIP/Postal code |
100871 |
| Country |
China |
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| Platforms (2) |
| GPL26171 |
HiSeq X Ten (Saccharomyces cerevisiae) |
| GPL29410 |
HiSeq X Ten ([Candida] glabrata) |
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| Samples (36)
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| Relations |
| SRA |
SRP291930 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE161373_cg_KCl_stress_rep1_featureCounts.csv.gz |
171.4 Kb |
(ftp)(http) |
CSV |
| GSE161373_cg_KCl_stress_rep2_featureCounts.csv.gz |
170.2 Kb |
(ftp)(http) |
CSV |
| GSE161373_sc_KCl_stress_rep1_fpkm.csv.gz |
502.2 Kb |
(ftp)(http) |
CSV |
| GSE161373_sc_KCl_stress_rep2_fpkm.csv.gz |
509.6 Kb |
(ftp)(http) |
CSV |
| GSE161373_sc_carbon_stress_rep1_fpkm.csv.gz |
410.7 Kb |
(ftp)(http) |
CSV |
| GSE161373_sc_carbon_stress_rep2_fpkm.csv.gz |
399.2 Kb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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