|
| Status |
Public on Apr 01, 2021 |
| Title |
Next generation sequencing of chicken otocyst with pharmacologicaly induced loss-of-Wnt signalling |
| Organism |
Gallus gallus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Bulk RNA-sequencing of individual E2.5 chicken otocyst incubated in media enriched with Wnt signalling inhibitor IWR-1 or control condition (DMSO) for 24h. Transcripts abundance was mapped to a chicken reference genome using Kallisto package and differentially expressed genes were identify using Sleuth package (genes with p-value < 0.05 were considered as significant). Biological Function enrichment analysis (Toppgene online tool) showed that many of these genes were associated with neurogenesis.
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| |
|
| Overall design |
Identification of genes deregulated in E3 otic explants treated with IRW-1 or DMSO for 24h
|
| |
|
| Contributor(s) |
Żak M, Daudet N |
| Citation missing |
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| |
| Submission date |
Nov 17, 2020 |
| Last update date |
Apr 03, 2021 |
| Contact name |
Magdalena Zak |
| E-mail(s) |
m.zak@ucl.ac.uk
|
| Organization name |
UCL Ear Institute
|
| Street address |
332 Grays Inn Road
|
| City |
London |
| ZIP/Postal code |
WC1X 8EE |
| Country |
United Kingdom |
| |
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| Platforms (1) |
| GPL19787 |
Illumina NextSeq 500 (Gallus gallus) |
|
| Samples (10)
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|
| Relations |
| BioProject |
PRJNA678981 |
| SRA |
SRP292928 |
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