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| Status |
Public on Nov 20, 2020 |
| Title |
MicroRNA profiling of cisplatin-resistant and cisplatin-sensitive ovarian cancer cells |
| Platform organism |
synthetic construct |
| Sample organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
Platinum resistance is a major drawback in the treatment of ovarian cancer. Evidence suggests that microRNAs are key players in the initiation, progression, and drug resistance of cancer cells. However, the precise miRNAs dysregulated and contributing to platinum resistance in ovarian cancer cells have not been fully elucidated. Here, we conducted a miRNA expression profiling of cisplatin-sensitive (A2780) and cisplatin-resistant (CP20 and CIS) ovarian cancer cells to identify potential miRNAs involved in platinum resistance.
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| |
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| Overall design |
Total RNA from ovarian cancer cells was isolated using the mirVana total RNA extraction kit. RNA was biotin-labeled and subjected to Affymetrix GeneChip miRNA 2.0 Arrays.
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| |
|
| Contributor(s) |
PE V |
| Citation(s) |
33392094 |
| |
| Submission date |
Nov 19, 2020 |
| Last update date |
Jan 19, 2021 |
| Contact name |
Pablo E Vivas-Mejía |
| E-mail(s) |
pablo.vivas@upr.edu
|
| Organization name |
University of Puerto Rico Cancer Center
|
| Department |
Cancer Medicine
|
| Lab |
Lab 8
|
| Street address |
Medical Sciences Campus
|
| City |
San Juan |
| State/province |
PR |
| ZIP/Postal code |
00935 |
| Country |
Puerto Rico |
| |
|
| Platforms (1) |
| GPL19117 |
[miRNA-4] Affymetrix Multispecies miRNA-4 Array |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA679457 |
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