|
| Status |
Public on Nov 21, 2020 |
| Title |
The small transcriptome of Escherichia coli in exponential and stationary phase |
| Organism |
Escherichia coli str. K-12 substr. MG1655 |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
We have deep sequenced the small transcriptome of Escherichia coli growing in LB and in MOPS, in exponential and stationary phase, and analyzed the resulting reads by a novel pipeline STARPA (Stable RNA Processing Product Analyzer). Our analysis reveals over 14,000 small transcripts enriched during both growth stages. RNA samples were collected from total RNA pools or from crude ribosome pools and then size selected by electrophoresis to limit the products to 20-300nt.
|
| |
|
| Overall design |
Differential expression analysis demonstrates the presence of enriched small transcripts in stationary phase, likely mediating regulatory functions.
|
| |
|
| Contributor(s) |
Hannes L, Nicole R, Michel F, Norbert P |
| Citation(s) |
33567722 |
| |
| Submission date |
Nov 20, 2020 |
| Last update date |
Feb 20, 2021 |
| Contact name |
Norbert Polacek |
| E-mail(s) |
ag.polacek@gmail.com, norbert.polacek@dcb.unibe.ch
|
| Organization name |
University of Bern
|
| Department |
Chemistry and Biochemistry
|
| Street address |
Freiestrasse 3
|
| City |
Bern |
| ZIP/Postal code |
3012 |
| Country |
Switzerland |
| |
|
| Platforms (1) |
| GPL15010 |
Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) |
|
| Samples (16)
|
|
| Relations |
| BioProject |
PRJNA679896 |
| SRA |
SRP294298 |
Less...
More...