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| Status |
Public on Jan 08, 2022 |
| Title |
Pharmacologic IRE1/XBP1s Activation Promotes Systemic Adaptive Remodeling in Obesity |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
RNA was isolated from livers of DIO mice treated with IXA4 or vehicle (n=4 per condition) using the Zymo Research Quick-RNA Miniprep Kit according to the manufacturer’s instructions. RNA sequencing was performed by BGI Americas on the BGI proprietary platform (DNBseq), providing single-end 50 bp reads at 20 million reads per sample. Alignment of the sequencing data to the Mus musculus MGSCv37 NCBI build 37.2 was performed using DNAstar Lasergene SeqManPro. Assembled data were then imported into ArrayStar 12.2 with QSeq (DNAStar Inc) to quantify gene expression and normalized reads per kilobase million (RPKM). Differential expression analysis and statistical significance calculations between conditions were assessed using DESeq in R using a standard negative binomial fit for the aligned counts data and are described relative to the indicated control
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| |
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| Overall design |
Comparisons of the transcriptomes of livers from DIO mice treated with or without IRE1 activator, IXA4.
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| Contributor(s) |
Madhavan A, Kok BP, Grandjean JD, Powers ET, Saez E, Wiseman RL |
| Citation(s) |
35105890 |
| |
| Submission date |
Dec 02, 2020 |
| Last update date |
Apr 09, 2022 |
| Contact name |
R. Luke Wiseman |
| Organization name |
The Scripps Research Institute
|
| Department |
Molecular Medicine
|
| Lab |
Wiseman
|
| Street address |
10550 North Torrey Pines Road
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92037 |
| Country |
USA |
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| Platforms (1) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA682221 |
| SRA |
SRP295565 |
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