|
| Status |
Public on Apr 20, 2021 |
| Title |
H3K36 methylation reprograms gene expression to drive early gametocyte development in Plasmodium falciparum |
| Organism |
Plasmodium falciparum NF54 |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
The Plasmodium sexual gametocyte stages are the only transmissible form of the malaria parasite and are thus responsible for the continued transmission of the disease. Gametocytes undergo extensive functional and morphological changes from commitment to maturity, directed by an equally extensive control program. Several interconnected mechanisms governing sexual commitment have been described. However, the processes that drive the subsequent differentiation and development of the gametocyte remain largely unexplored. Using chromatin immunoprecipitation followed by high-throughput sequencing we map the genome-wide occupancy of H3K36me2 and H3K36me3 during early gametocyte development and describe an association between these histone modifications and the global changes in the transcriptional program driving gametocyte development post-commitment.
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| |
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| Overall design |
Investigation of H3K36me2 and H3K36me3 dynamics during early development in P. falciparum gametocytes
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| |
|
| Contributor(s) |
Connacher J, Josling G, Orchard L, Reader J, Llinas M, Birkholtz L |
| Citation(s) |
33794978 |
| |
| Submission date |
Dec 17, 2020 |
| Last update date |
Apr 20, 2021 |
| Contact name |
Jessica I Connacher |
| E-mail(s) |
jessica.connacher@gmail.com
|
| Phone |
0718803047
|
| Organization name |
University of Pretoria
|
| Street address |
Lynnwood road, Hatfield
|
| City |
Pretoria |
| ZIP/Postal code |
0002 |
| Country |
South Africa |
| |
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| Platforms (1) |
| GPL29510 |
Illumina HiSeq 2500 (Plasmodium falciparum NF54) |
|
| Samples (17)
|
| GSM4979231 |
H3K36me2 in pre-stage II gametocytes_rep1 |
| GSM4979232 |
H3K36me3 in pre-stage II gametocytes_rep1 |
| GSM4979233 |
Input for pre-stage II gametocytes_rep1 |
| GSM4979234 |
H3K36me2 in pre-stage II gametoytes_rep2 |
| GSM4979235 |
Input for pre-stage II gametocytes_rep2 |
| GSM4979236 |
H3K36me2 in stage II gametocytes_rep1 |
| GSM4979237 |
H3K36me3 in stage II gametocytes_rep1 |
| GSM4979238 |
Input for stage II gametocytes_rep1 |
| GSM4979239 |
H3K36me2 in stage II gametocytes_rep2 |
| GSM4979240 |
H3K36me3 in stage II gametocytes_rep2 |
| GSM4979241 |
Input for stage II gametocytes_rep2 |
| GSM4979242 |
H3K36me2 in post-stage II gametoytes_rep1 |
| GSM4979243 |
H3K36me3 in post-stage II gametoytes_rep1 |
| GSM4979244 |
Input for post-stage II gametocytes_rep1 |
| GSM4979245 |
H3K36me2 in post-stage II gametoytes_rep2 |
| GSM4979246 |
H3K36me3 in post-stage II gametoytes_rep2 |
| GSM4979247 |
Input for post-stage II gametocytes_rep2 |
|
| Relations |
| BioProject |
PRJNA686098 |
| SRA |
SRP298345 |
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