|
| Status |
Public on Jan 01, 2021 |
| Title |
Re-expression of SMARCA4/BRG1 in Small Cell Carcinoma of Ovary, Hypercalcemic Type (SCCOHT) promotes an epithelial-like gene signature through an AP-1-dependent mechanism [CCOHT1_RNA-seq] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
SCCOHT-1 cells +/- BRG1 re-expression - RNA-seq
|
| |
|
| Overall design |
Three biological replicates of SCCOHT-1 cells were co-transfected (FuGENE 6, Promega #E269A) with control (pCDNA3-neo) or BRG1 (PBJ5-BRG1) plasmids at a 6.25:1 ratio to a plasmid containing puromycin resistance (pBABE-puro). Twenty-four hours after transfection, cells were placed on 0.5 µg/ml puromycin for 3 days and then cell pellets were harvested for RNA-seq.
|
| |
|
| Contributor(s) |
Orlando KA, Weissman BE, Raab JR |
| Citation(s) |
33355532 |
| |
| Submission date |
Dec 31, 2020 |
| Last update date |
Apr 02, 2021 |
| Contact name |
Bernard E. Weissman |
| E-mail(s) |
weissman@med.unc.edu
|
| Organization name |
University of North Carolina at Chapel Hill
|
| Department |
Pathology and Laboratory Medicine
|
| Street address |
450 West Drive
|
| City |
Chapel Hill |
| State/province |
NC |
| ZIP/Postal code |
27599 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
|
| Samples (6)
|
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| This SubSeries is part of SuperSeries: |
| GSE151026 |
Re-expression of SMARCA4/BRG1 in Small Cell Carcinoma of Ovary, Hypercalcemic Type (SCCOHT) promotes an epithelial-like gene signature through an AP-1-dependent mechanism |
|
| Relations |
| BioProject |
PRJNA688937 |
| SRA |
SRP299936 |
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