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| Status |
Public on Jul 31, 2021 |
| Title |
Chromatin remodeling and expression dynamics reveal conserved and species-specific regulation during mouse and chicken limb development [RNA-seq] |
| Organisms |
Gallus gallus; Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Understanding the origin of morphological diversity across vertebrates is central to evolutionary developmental biology. cis-regulatory elements (CRE) such as enhancers and promoters interpret precise spatiotemporal cues to control and coordinate gene expression. To get insights into both conserved and species-specific variations during early limb patterning and outgrowth, we leverage genome-wide comprehensive assessment of chromatin accessibility and transcriptional changes during mouse forelimb and chicken wing bud development. Our analysis reveals temporal modulation of chromatin accessibility and expression as well as their temporal relationship during the progression of mouse forelimb and chicken wing bud development. Transcription factor binding site enrichment analysis and putative TF occupancy as inferred by integrating TF binding motifs and chromatin accessibility information reveal temporal TF-DNA interactions during forelimb/wing bud patterning. Finally, the integration of accessibility, expression, and TF binding site information allowed to identify candidate gene targets of HAND2 and GLI3 that include conserved as well as species-specific transcriptional regulator-gene interactions.
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| |
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| Overall design |
RNA-seq analysis of developing mouse forelimb and chicken wing buds
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| |
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| Contributor(s) |
Jhanwar S, Zuniga A, Zeller R |
| Citation(s) |
34584102 |
| |
| Submission date |
Jan 13, 2021 |
| Last update date |
Oct 27, 2021 |
| Contact name |
Shalu Jhanwar |
| E-mail(s) |
shalu.jhanwar@unibas.ch
|
| Organization name |
University of Basel
|
| Department |
Department of Biomedicine
|
| Street address |
Mattenstrasse 28
|
| City |
Basel |
| ZIP/Postal code |
4058 |
| Country |
Switzerland |
| |
|
| Platforms (2) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
| GPL19787 |
Illumina NextSeq 500 (Gallus gallus) |
|
| Samples (18)
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| This SubSeries is part of SuperSeries: |
| GSE164738 |
Chromatin remodeling and expression dynamics reveal conserved and species-specific regulation during mouse and chicken limb development |
|
| Relations |
| BioProject |
PRJNA691876 |
| SRA |
SRP301589 |
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