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| Status |
Public on Feb 10, 2021 |
| Title |
Single cell analysis of the ventricular-subventricular zone reveals signatures of dorsal and ventral adult neurogenic lineages. [single nucleus sequencing] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Purpose: While great progress has been made in understanding the differences in regional stem cell potential using viral and genetic lineage tracing strategies, the core molecular heterogeneity that underlies these regional differences is largely unknown.
Methods: Here we present a single nucleus sequencing dataset of four microdissected regions of adult CD1 wild type mouse ventricular-subventricular zones (V-SVZ). Four samples (anterior-ventral, AV; anterior dorsal, AD; posterior ventral, PV; posterior dorsal, PD) were generated at the same time from the same mice, and each sample was run on its own lane of a 10x Chromium Single Cell Controller chip for single nucleus barcoding.
Results: Here we present single nucleus and single whole cell sequencing datasets of microdissected adult mouse V-SVZ, and evidence for the existence of two broad subtypes of adult neural stem cells. By using spatially resolved microdissections in the single nucleus sequencing dataset as a reference, and mapping marker gene expression in the V-SVZ, we find that these two populations reside in largely non-overlapping domains in either the dorsal or ventral V-SVZ. Furthermore, we identified two subpopulations of newly born neurons that have gene expression consistent with dorsal or ventral origins. Finally, we identify genes expressed by both stem cells and the neurons they generate that specifically mark either the dorsal or ventral adult neurogenic lineage. These datasets, methods and findings will enable future study of region-specific regulation of adult neurogenesis.
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| Overall design |
Single nucleus profiles of regionally micro-dissected ventricular-subventricular zones of 17 postnatal day 35 male (8 mice) and female (9 mice) CD1 wild type mice.
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| Contributor(s) |
Redmond SA, Cebrian Silla A, AssisNascimento M, Mansky B, Wu D, Obernier K, Romero Rodriguez R, Lim DA, Alvarez-Buylla A |
| Citation(s) |
34259628 |
| |
| Submission date |
Jan 26, 2021 |
| Last update date |
Dec 01, 2023 |
| Contact name |
Stephanie A Redmond |
| E-mail(s) |
stephanie.redmond@ucsf.edu
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| Organization name |
University of California San Francisco
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| Department |
Neurosurgery
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| Lab |
Arturo Alvarez-Buylla
|
| Street address |
35 Medical Center Way, 933D Box 0525
|
| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94143 |
| Country |
USA |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE165555 |
Single cell analysis of the ventricular-subventricular zone reveals signatures of dorsal and ventral adult neurogenic lineages. |
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| Relations |
| BioProject |
PRJNA694946 |
| SRA |
SRP303343 |
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