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| Status |
Public on Feb 25, 2010 |
| Title |
Gene expression analysis of ovarian serous adenocarcinoma cell lines and tumors |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
A variety of human cancers demonstrate alterations in microRNA expression. We hypothesized that regulatory defects in microRNAs play a central early role in organizing the molecular changes involved in ovarian cancer (OvCa). Using both gene arrays and deep sequencing, we comprehensively profiled mRNA and microRNA expression, respectively, in human serous epithelial OvCa cell lines, serous tumors, and short-term primary cultures of normal ovarian surface epithelium (NOSE). We expected that over-expression of a specific microRNA would lead to lower expression of its mRNA targets, and under-expression of a specific microRNA would lead to higher expression of its target genes. Using our expression data in conjunction with established in silico algorithms, we found putative microRNA:mRNA functional pairs.
Keywords: two group comparison
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| Overall design |
We generated gene expression profiles of a panel of seven OvCa serous cell lines, 17 serous tumors, and nine independent normal ovarian surface epithelium (NOSE) cultures. We defined genes that were high or low in both cell lines and tumors relative to NOSE.
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| Contributor(s) |
Creighton C, Matzuk M, Gunaratne P, Anderson M, Nagaraja A, Fountain M |
| Citation(s) |
20179198 |
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| Submission date |
Jun 18, 2009 |
| Last update date |
Aug 16, 2018 |
| Contact name |
Chad Creighton |
| E-mail(s) |
creighto@bcm.tmc.edu
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| Organization name |
Baylor College of Medicine
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| Department |
Biostatistics, Ducan Cancer Center
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| Street address |
One Baylor Plaza, Mail Stop: BCM305
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| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
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| Platforms (1) |
| GPL6947 |
Illumina HumanHT-12 V3.0 expression beadchip |
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| Samples (33)
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| GSM418627 |
NOSE cell culture, biological rep4 |
| GSM418628 |
NOSE cell culture, biological rep5 |
| GSM418629 |
NOSE cell culture, biological rep6 |
| GSM418630 |
NOSE cell culture, biological rep7 |
| GSM418631 |
NOSE cell culture, biological rep8 |
| GSM418632 |
NOSE cell culture, biological rep9 |
| GSM418633 |
OVCA-433 serous cell line culture, subconfluent |
| GSM418634 |
SKOV3 serous cell line culture, subconfluent |
| GSM418635 |
OV-90 serous cell line culture, subconfluent |
| GSM418636 |
OVCAR-3 serous cell line culture, subconfluent |
| GSM418637 |
HEY serous cell line culture, subconfluent |
| GSM418638 |
OVCAR-5 serous cell line culture, subconfluent |
| GSM418639 |
OVCAR-8 serous cell line culture, subconfluent |
| GSM418640 |
serous tumor (39395) |
| GSM418641 |
serous tumor (39513) |
| GSM418642 |
serous tumor (39245) |
| GSM418643 |
serous tumor (human_15_b) |
| GSM418644 |
serous tumor (11888) |
| GSM418645 |
serous tumor (16399) |
| GSM418646 |
serous tumor (9418) |
| GSM418647 |
serous tumor (9510) |
| GSM418648 |
serous tumor (10367) |
| GSM418649 |
serous tumor (13396) |
| GSM418650 |
serous tumor (1459) |
| GSM418651 |
serous tumor (1576) |
| GSM418652 |
serous tumor (18132) |
| GSM418653 |
serous tumor (2386) |
| GSM418654 |
serous tumor (1198) |
| GSM418655 |
serous tumor (15989) |
| GSM418656 |
serous tumor (21027) |
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| This SubSeries is part of SuperSeries: |
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| Relations |
| BioProject |
PRJNA122689 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE16708_RAW.tar |
28.0 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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