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| Status |
Public on Jun 01, 2022 |
| Title |
Transcriptional responses to maternal trichloroethylene and/or N-acetyl cysteine exposure in Wistar rat placental tissue |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Trichloroethylene (TCE) is a persistent and pervasive environmental contaminant. N-acetyl cysteine (NAC) is an antioxidant that may reduce TCE effects. Pregnant Wistar rats were exposed to 480 mg TCE mg/kg/day, 200 mg/kg/day NAC or co-exposure with both chemicals via ingestion (mini vanilla wafer) on gestation days 6-16 (tissue collection day). TCE- and/or NAC-induced changes to gene expression were evaluated in male and female rat placentae.
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| Overall design |
Timed-pregnant Wistar rats were exposed to vehicle control (PBS), trichloroethylene (TCE), N-acetyl cysteine (NAC) or co-exposure with both chemicals via ingestion (treated mini vanilla wafer) on gestation days 6-16 (tissue collection day). The doses were as follows: 480 mg TCE mg/kg/day, 200 mg/kg/day NAC or both doses together. There were 6 batches of rats (N=6) with 4 rats in each batch and 1 rat assigned to each treatment group (total of 24 dams). RNA was isolated from one male and one female placenta from each respective dam and sequenced (24 male and 24 female placental tissue samples). Differential gene expression comparisons were made between each treatment group and control tissues stratified by sex.
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| Contributor(s) |
Elkin ER, Bakulski KM, Su AL, Dou JF, Loch-Caruso R |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Mar 04, 2021 |
| Last update date |
Jun 01, 2022 |
| Contact name |
Elana Elkin |
| Organization name |
University of Michigan
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| Department |
Department of Environmental Health Sciences
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| Lab |
Bakulski
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| Street address |
1415 Washington Heights
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| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48109 |
| Country |
USA |
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| Platforms (1) |
| GPL25947 |
Illumina NovaSeq 6000 (Rattus norvegicus) |
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| Samples (48)
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| Relations |
| BioProject |
PRJNA706597 |
| SRA |
SRP309262 |
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