|
| Status |
Public on Nov 19, 2021 |
| Title |
Genome-scale CRISPR screen identifies TMEM41B as a critical host factor required for coronavirus replication [PigGeCKO] |
| Organism |
Sus scrofa |
| Experiment type |
Other
|
| Summary |
We use the a porcine genome-scale CRISPR/Cas9 knockout (PigGeCKO) library (Zhao et al., 2020) to identify key host factors facilitating TGEV infection in porcine cells.
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| |
|
| Overall design |
We generated a PigGeCKO cell collection (in the PK-15 background) as previously described and performed a genome-wide loss-of-function genetic screen.Briefly, we conducted three rounds of TGEV challenge, employing untreated PK-15-Cas9 cells as a negative control.
|
| Web link |
https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1010113
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| |
|
| Contributor(s) |
Peng G, Sun L, Xie S, Zhao C |
| Citation(s) |
34871328 |
| |
| Submission date |
Mar 17, 2021 |
| Last update date |
Feb 22, 2022 |
| Contact name |
Shengsong Xie |
| E-mail(s) |
ssxie@sibcb.ac.cn
|
| Organization name |
Huazhong Agricultural University
|
| Street address |
1st Shizishan Street, Hongshan
|
| City |
Wuhan |
| State/province |
Hubei |
| ZIP/Postal code |
430070 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL24486 |
Illumina HiSeq 3000 (Sus scrofa) |
|
| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE169113 |
Genome-scale CRISPR screen identifies TMEM41B as a critical host factor required for coronavirus replication |
|
| Relations |
| BioProject |
PRJNA715228 |
| SRA |
SRP311102 |
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