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| Status |
Public on Sep 20, 2022 |
| Title |
Single nuclei RNAseq of rat area nucleus accumbens after morphine exposure |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We performed single-nuclei RNAseq of Brown Norway rat nucleus accumbens after a single injection of morphine or after morhpine self-administration
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| Overall design |
We performed the 10x Genomics 3' gene expression assay (v3.1) on nuclei isolated from frozen Brown Norway (BN) rat nucleus accumbens brain punches. A cohort of male BN rats received an experimenter delivered dose of morphine (10.0 mg/kg, ip) or saline (n=5 each) and were sacrificed two hours after injection. A second cohort of male BN rats underwent volitional IV morphine self-administration (1.0mg/kg/infusion) for three hours daily for 10 days, along with their saline yoked controls (n=6 each), and animals were sacrificed two hours after their final self-administration session. Brains were removed and snap-frozen in dry ice-cold isopentane. Brains were coronally sectioned and the nucleus accumbens core and shell was removed by micro-punch bilaterally, and the punches were stored at -80°C until further processing. Nuclei were isolated by differential centrifugation and microfluidically captured using the 10x Genomics Chromium controller. Sequencing libraries were constructed and sequenced on an Illumina NovaSeq 6000.
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| Contributor(s) |
Reiner BC, Crist RC, Berrettini WH, Schmidt H |
| Citation(s) |
36075888 |
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| Submission date |
Mar 30, 2021 |
| Last update date |
Apr 05, 2024 |
| Contact name |
Benjamin C. Reiner |
| E-mail(s) |
bcreiner@pennmedicine.upenn.edu
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| Organization name |
University of Pennsylvania
|
| Street address |
125 S 31st St
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| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
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| Platforms (1) |
| GPL25947 |
Illumina NovaSeq 6000 (Rattus norvegicus) |
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| Samples (22)
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| Relations |
| BioProject |
PRJNA718575 |
| SRA |
SRP312753 |
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