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| Status |
Public on Jul 20, 2021 |
| Title |
Timecourse RNAseq for Drosophila TBI |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Comparison of post-injury (sham versus severe TBI) gene expression changes using a Drosophila head-specific TBI model.
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| Overall design |
64 total libraries were prepared from 52 unique RNA samples, representing the molecular response to traumatic brain injury spanning 9 timepoints from 0 through 15 days post-injury. Samples consist of heads isolated from 3d old uninjured males (controls, 0 post injury timepoint) and paired sham vs TBI injured animals (3d old males) at 1h, 3h, 6h, 12h, 24h, 3d, 7d, 10d, and 15d post-injury. All times/conditions have 3 biological replicates, with the exception of 15d due to library prep failure. Library preparation took place on two separate days (noted as library 1 or library 2), hence libraries for control and sham/TBI 24 h timepoints were prepared twice and used for batch effect correct.
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| Contributor(s) |
Byrns CN, Bonini NM |
| Citation missing |
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| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| T32 AG000255 |
Training in Alzheimer?s and Age-Related Neurodegenerative Diseases |
UNIVERSITY OF PENNSYLVANIA |
VIRGINIA M LEE |
| R35 NS097275 |
Molecular Genetic Insight into Neurodegenerative Disease from Drosophila |
UNIVERSITY OF PENNSYLVANIA |
Nancy M Bonini |
| F31 NS111868 |
Novel mechanisms of the glial response to traumatic brain injury: dissecting the role of Ets21c in a Drosophila model |
UNIVERSITY OF PENNSYLVANIA |
China Byrns |
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| Submission date |
Mar 30, 2021 |
| Last update date |
Jul 20, 2021 |
| Contact name |
Nancy M Bonini |
| Organization name |
University of Pennsylvania
|
| Street address |
433 South University Ave
|
| City |
Philadelphia |
| ZIP/Postal code |
19104 |
| Country |
USA |
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| Platforms (1) |
| GPL19132 |
Illumina NextSeq 500 (Drosophila melanogaster) |
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| Samples (64)
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| Relations |
| BioProject |
PRJNA718601 |
| SRA |
SRP312776 |
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