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| Status |
Public on Jul 23, 2010 |
| Title |
Direct effects of IFNα on human CD8 T cells_without any other concomitant signals |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
IFNα-mediated gene expression pattern. Direct effects of IFNα on human CD8 T cells without any other concomitant signal.
This analysis examined the direct effects of IFNa on human CD8 T cells without any other concomitant signal. Total human CD8 T cells were magnetically sorted from peripheral blood by negative selection. In order to minimize the presence of antigen-experienced cells, total human CD8 T cells were depleted of CD45RO+ cells by labelling with anti-CD45RO magnetic bead. In both steps of cellular sorting, negative selection were preferred to positive selection to avoid direct labelling of CD8 T cell surface molecules could interfere with downstream IFNa-mediated signalling. Magnetically sorted untouched human CD8+CD45R0- T cells from three different donors were unstimulated or stimulated with IFNa2b or IFNa5 and cells were harvested 7 hours later. mRNAs from the 3 subjects were pooled and analyzed using Affymetrix human HG-U133A 2.0 array gene chips Fold-change ≥ 2 criterion was used to identify genes which expression differed between treatment and control (unstimulated cells).
Keywords: Gene expression pattern analysis
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| Overall design |
Magnetically sorted untouched CD8+CD45R0-T cells mRNA pooled sample from three different donors unstimulated or stimulated with IFN alfa 2b or IFN alfa 5 for 7 hours.
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| Contributor(s) |
Hervás-Stubbs S, Riezu JI, González I, Mancheño U, Dubrot J, Azpilicueta A, Gabari I, Palazón A, Esquiroz P, Ruiz J, Prieto J, Le Bon A, Corrales F, Larrea E, Melero I |
| Citation missing |
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| |
| Submission date |
Jul 24, 2009 |
| Last update date |
Dec 06, 2018 |
| Contact name |
Iranzu Gonzalez |
| URL |
http://www.cima.es
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| Organization name |
Center for applied medical research
|
| Department |
Hepathology and Gene Therapy
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| Lab |
3.03. Clinic immunology
|
| Street address |
Avda. Pio XII nº 55
|
| City |
Pamplona |
| State/province |
Navarra |
| ZIP/Postal code |
31008 |
| Country |
Spain |
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| Platforms (1) |
| GPL571 |
[HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array |
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| Samples (3) |
| GSM432923 |
Magnetically sorted untouched CD8+CD45R0-T cells mRNA from a three donors pooled sample unstimulated |
| GSM432924 |
Magnetically sorted untouched CD8+CD45R0-T cells mRNA from a three donors pooled sample stimulated 7 hours with IFN α2 |
| GSM432925 |
Magnetically sorted untouched CD8+CD45R0-T cells mRNA from a three donors pooled sample stimulated 7 hours with IFN α5 |
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| This SubSeries is part of SuperSeries: |
| GSE17302 |
IFNα-mediated gene expression patterns |
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| Relations |
| BioProject |
PRJNA123601 |
