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| Status |
Public on Sep 14, 2021 |
| Title |
Reactivation of Tumor Suppressor in Breast Cancer by Enhancer Switching through NamiRNA Network |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We reported that low expression of miRNA in cancer as a recognized signature leads to loss function of TSGs in breast cancer. In 157 paired breast cancer and adjacent normal samples, tumour suppressor gene GPER1 and miR-339 are both downregulated in Luminal A/B and Triple Negative Breast Cancer subtypes. Mechanistic investigations revealed that that miR-339 upregulates GPER1 expression in breast cancer cells by switching on the GPER1 enhancer, which can be blocked by enhancer deletion through the CRISPR/Cas9 system.
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| |
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| Overall design |
1). Examination of H3K27ac histone modification alterations in 293T cells after overexpressing miR-339; 2). mRNA profiles of 4175 cells after overexpressing miR-339
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| |
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| Contributor(s) |
Liang Y, Wang S, Li W, Li W, Yu W |
| Citation(s) |
34329471 |
| |
| Submission date |
May 06, 2021 |
| Last update date |
Sep 15, 2021 |
| Contact name |
Ying Liang |
| E-mail(s) |
16111510047@fudan.edu.cn
|
| Phone |
18717906439
|
| Organization name |
Fudan University
|
| Street address |
Room 416, West Building 13, Dong'an Road 130, Xuhui District
|
| City |
Shanghai |
| State/province |
Shanghai |
| ZIP/Postal code |
200032 |
| Country |
China |
| |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (5)
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| Relations |
| BioProject |
PRJNA727844 |
| SRA |
SRP318785 |
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