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Series GSE174253 Query DataSets for GSE174253
Status Public on Dec 27, 2022
Title The molecular programs underpinning 'tolerance' and 'resistance' in health and viral infection
Organisms Mus musculus; Influenza A virus (A/Puerto Rico/8/1934(H1N1))
Experiment type Expression profiling by high throughput sequencing
Summary The immune protection against invading pathogens is governed by pathogen elimination (‘resistance’) and by limitation of tissue damage that results from host-pathogen interactions (‘tolerance’). However, distinct molecular states of resistance and tolerance remain obscure. Here, we collected longitudinal lung transcriptomes and clinical phenotypes of disease severity during influenza A virus (IAV) infection across 39 genetically-distinct mouse strains (32 strains of time series data and additional 7 strains with selected time points). Using transcriptional diversity in this data, we uncovered two key programs that together describe the cellular states for resistance and tolerance. The identified programs offer an unprecedented comprehensive view on shared and distinct roles of resistance and tolerance for the regulation of immune defense – for instance, we identified an antagonistic regulation of resistance and tolerance on protein production. Importantly, the framework reveals that both inflammatory states and the healthy steady state are remarkably diverse in resistance and tolerance levels, with implications on disease severity: a baseline state (before infection) of high tolerance and low resistance results in increased severity of IAV infection. Overall, our work provides a framework for the study of resistance and tolerance that could aid in clinical diagnosis and disease management.
 
Overall design To characterize the host defense, we investigated longitudinal data during IAV infection across genetically distinct mouse strains. We recorded lung transcriptomes of mouse strains of the collaborative cross (CC) cohort, both in steady state (before infection) and at five time points during the course of IAV infection (3h to 96h p.i.).
 
Contributor(s) Cohn O, Yankovitz G, Peshez-Yaloz N, Steuerman Y, Frishberg A, Mandelboim M, Iraqi F, Bacharach E, Gat-Viks I
Citation(s) 36516834
Submission date May 11, 2021
Last update date Dec 27, 2022
Contact name Gal Yankovitz
E-mail(s) galyankovitz@mail.tau.ac.il
Organization name Tel-Aviv University
Department The Shmunis School of Biomedicine and Cancer Research
Lab Gat-Viks
Street address Haim Levanon
City Tel-Aviv
ZIP/Postal code 69978
Country Israel
 
Platforms (1)
GPL30115 Illumina HiSeq 2500 (Influenza A virus (A/Puerto Rico/8/1934(H1N1)); Mus musculus)
Samples (198)
GSM5289969 3 hours post IAV infection, strain 1912A
GSM5289970 3 hours post IAV infection, strain 72A
GSM5289971 3 hours post IAV infection, strain 2126A
Relations
BioProject PRJNA728967
SRA SRP319478

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE174253_exp_all_time_points.csv.gz 8.0 Mb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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