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| Status |
Public on Nov 04, 2009 |
| Title |
Global gene-expression analyses of the Eset knock-down ES cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The histone H3 lysine 9 (H3K9) methyltransferase Eset is an epigenetic regulator critical for the development of the inner cell mass (ICM). Although ICM-derived embryonic stem (ES) cells are normally unable to contribute to the trophectoderm (TE) in blastocysts, we find that depletion of Eset by shRNAs leads to differentiation with the formation of trophoblast-like cells and induction of trophoblast-associated gene expression. Using ChIP-seq analyses, we identified Eset target genes with Eset-dependent H3K9 trimethylation. We confirmed that genes that are preferentially expressed in the TE (Tcfap2a and Cdx2) are bound and repressed by Eset. Single cell PCR analysis shows that the expression of Cdx2 and Tcfap2a is also induced in Eset-depleted morula cells. Importantly, Eset-depleted cells can incorporate into the TE of a blastocyst and subsequently placental tissues. Co-immunoprecipitation and ChIP assays further demonstrates that Eset interacts with Oct4, which in turn recruits Eset to silence these trophoblast-associated genes. Our result suggests that Eset restricts the extraembryonic trophoblast lineage potential of pluripotent cells and links an epigenetic regulator to key cell fate decision through a pluripotency factor.
We used microarrays to detail the global programme of gene expression of control shRNA knock-down ES cells and Eset shRNA knock-down ES cells
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| Overall design |
Three biological replicates for control shRNA knock-down ES cells and Eset knock-down ES cells. The global gene expression profiles of the control shRNA knock-down ES cells were compared to Eset knock-down ES cells.
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| Contributor(s) |
Yuan P, Han J, Guoji G, Orlov Y, Huss M, Loh YH, Clarke N, Robson P, Lin B, Ng HH |
| Citation(s) |
19884257 |
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| Submission date |
Jul 30, 2009 |
| Last update date |
Mar 21, 2012 |
| Contact name |
Ping Yuan |
| E-mail(s) |
yuanp@gis.a-star.edu.sg
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| Phone |
(65)64788144
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| Fax |
(65)64789004
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| Organization name |
Genome Institute of Singapore
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| Department |
Stem Cell
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| Lab |
Stem Cell 6
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| Street address |
60 Biopolis Street #02-01
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| City |
Singapore |
| ZIP/Postal code |
138672 |
| Country |
Singapore |
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| Platforms (1) |
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| Samples (6)
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| GSM435059 |
Control shRNA ES cells, biological replicate 1 |
| GSM435060 |
Control shRNA ES cells, biological replicate 2 |
| GSM435061 |
Control shRNA ES cells, biological replicate 3 |
| GSM435062 |
Eset shRNA ES cells, biological replicate 1 |
| GSM435063 |
Eset shRNA ES cells, biological replicate 2 |
| GSM435064 |
Eset shRNA ES cells, biological replicate 3 |
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| Relations |
| BioProject |
PRJNA118881 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE17439_EsetKnockDownRawData.txt.gz |
3.0 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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