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| Status |
Public on Dec 01, 2021 |
| Title |
S100A8/A9 induced by interaction with macrophages in esophageal squamous cell carcinoma promotes the migration and invasion of cancer cells via Akt and p38 MAPK pathways |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Tumor-associated macrophages enhance the malignant phenotypes of esophageal squamous cell carcinoma (ESCC) cells. We have previously identified several factors associated with ESCC progression using an indirect co-culture assay between ESCC cells and macrophages. Here, we newly established a direct co-culture assay between ESCC cells and macrophages which is closer to the actual cancer microenvironment than an indirect co-culture assay. To investigate the gene expression changes by co-culture with macrophages, we performed cDNA microarray analysis between mono-cultured and co-cultured ESCC cells with macrophages. We found that the expression of S100 calcium binding protein A8 and A9 (S100A8 and S100A9) was enhanced in co-cultured ESCC cells with macrophages.
S100A8 and S100A9 commonly exist stable and function as a heterodimer (S100A8/A9). S100A8/A9 is widely known as an inflammation marker. It also contributes to the enhancement of malignant phenotypes in several cancers. S100A8/A9 enhances the migration and invasion of ESCC cells by activating Akt and p38 MAPK signaling pathways. The higher expression levels of S100A8/A9 were associated with poor prognosis in ESCC patients. These results suggest that S100A8/A9 contributes to the progression of ESCC.
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| Overall design |
CD14 positive peripheral blood monocytes (PBMos) were selectively collected from healthy volunteers. Recombinant human macrophage colony stimulating factor (rhM-CSF) was added to induce M2 macrophages. We seeded ESCC cell line (TE-11) and direct co-cultured with the M2 macrophages. As a control, the same amount of TE-11 without macrophage was seeded and mono-cultured. We performed cDNA microarray analysis between mono-cultured TE-11 cells and co-cultured TE-11 cells with macrophages.
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| Contributor(s) |
Tanigawa K, Koma YI |
| Citation(s) |
34954212 |
| Submission date |
May 20, 2021 |
| Last update date |
Jun 27, 2022 |
| Contact name |
Satoshi Kondo |
| Organization name |
Toray Industries,Inc.
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| Street address |
10-1 tebiro 6-chome
|
| City |
Kamakura |
| State/province |
Kanagawa |
| ZIP/Postal code |
248-8555 |
| Country |
Japan |
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| Platforms (1) |
| GPL22439 |
3D-Gene Human Oligo chip 25k V2.2.0 |
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| Samples (1) |
| GSM5328311 |
mono-cultured TE-11 vs direct co-cultured TE-11 with macrophages |
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| Relations |
| BioProject |
PRJNA731538 |
