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| Status |
Public on Mar 31, 2010 |
| Title |
Detection of aberrant DNA methylation in colorectal carcinoma samples compared to normal human colon |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by genome tiling array
Genome variation profiling by genome tiling array
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| Summary |
To globally define methylation-’prone’ and -’protected’ CpG islands in colorectal carcinoma we analyzed the methylation status of 23,000 CpG islands of the human genome in ten coleorectal carcinoma samples as well as normal colon using our previously described methyl-CpG immunoprecipitation (MCIp) technique (Gebhard et al. 2006; Schilling and Rehli 2007). This method enriches for highly CpG methylated DNA that can be directly applied to fluorescent labeling and oligonucleotide microarray hybridization without an additional amplification step.
Keywords: MCIp-on-Chip; comparative genomic hybridization
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| Overall design |
CpG-methylated genomic DNA was enriched using methyl-CpG immunoprecipitation (MCIp). On each microarray, the enriched material from colorectal carcinoma samples was compared to the enriched material from normal colon to identify aberrantly methylated regions.
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| Contributor(s) |
Gebhard C, Rehli M |
| Citation(s) |
20145141 |
| |
| Submission date |
Aug 05, 2009 |
| Last update date |
Jan 17, 2013 |
| Contact name |
Michael Rehli |
| E-mail(s) |
michael.rehli@klinik.uni-r.de
|
| Organization name |
University Hospital Regensburg
|
| Department |
Internal Med III
|
| Street address |
F.-J.-Strauss-Allee 11
|
| City |
Regensburg |
| ZIP/Postal code |
93042 |
| Country |
Germany |
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| Platforms (1) |
| GPL8544 |
Human CpG Island Microarray 244K (Agilent-014791) with extended custom annotation |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA118783 |
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