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| Status |
Public on Jun 15, 2021 |
| Title |
Dynamic imaging of nascent RNA reveals general principles of transcription dynamics and pervasive recursive splicing [pulse_chase_nascent RNA] |
| Organism |
Homo sapiens |
| Experiment type |
Other
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| Summary |
The coupled activities of RNA polymerase and the spliceosome are responsible for the abundance and isoform composition of mRNA in human cells. However, the dynamics of these megadalton enzymatic complexes working in concert on endogenous genes have not been described. Here, we establish a quasi-genome-scale platform for observing synthesis and processing kinetics of single nascent RNA molecules in real time. We find that all observed genes show transcriptional bursting. We also observe large kinetic variation in intron removal for single introns in single cells which is inconsistent with deterministic splice site selection. Transcriptome-wide footprinting of the U2AF complex further reveals widespread splice site selection within introns. We propose and validate a unified theoretical model for transcription and splicing based on pervasive stochastic recursive splicing.
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| Overall design |
Pulse Chase Nascent RNA profiling in HBEC cells
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| Contributor(s) |
Wan Y, Larson DR |
| Citation(s) |
33979654 |
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| Submission date |
Jun 14, 2021 |
| Last update date |
Sep 14, 2021 |
| Contact name |
Daniel R Larson |
| E-mail(s) |
yihan.wan@nih.gov
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| Organization name |
NIH
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| Street address |
41 Medlar Dr.
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| City |
Bethesda |
| ZIP/Postal code |
20892 |
| Country |
USA |
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| Platforms (1) |
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| Samples (6)
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| GSM5381369 |
HBECs, pulse chase Nascent RNA-seq, rep_A_10min |
| GSM5381370 |
HBECs, pulse chase Nascent RNA-seq, rep_A_20min |
| GSM5381371 |
HBECs, pulse chase Nascent RNA-seq, rep_A_40min |
| GSM5381372 |
HBECs, pulse chase Nascent RNA-seq, rep_B_5min |
| GSM5381373 |
HBECs, pulse chase Nascent RNA-seq, rep_B_10min |
| GSM5381374 |
HBECs, pulse chase Nascent RNA-seq, rep_B_20min |
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| This SubSeries is part of SuperSeries: |
| GSE134377 |
Dynamic imaging of nascent RNA reveals general principles of transcription dynamics and pervasive recursive splicing |
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| Relations |
| BioProject |
PRJNA737562 |
| SRA |
SRP324043 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE178182_Yihan_GEO_nascent_RNA_pulse_chase_processed_data.xlsx |
2.0 Mb |
(ftp)(http) |
XLSX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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