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| Status |
Public on Apr 30, 2023 |
| Title |
Endothelial cells synergize with fibroblasts to form a niche for engineering alveolar epithelium |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We perform single-cell RNA sequencing of whole engineered lungs generated either by alveolar epithelial type 2 cell (AEC2)/fibroblast (FB) co-culture or by AEC2/FB/endothelial cell (EC) tri-culture, to investigate AEC2 and FB phenotype in the presence and absence of endothelial cells. Primary postnatal day 7 (P7) AEC2s, P7 lung FBs, and primary rat lung microvascular ECs (from 4-6 week-old rats) used for engineered cultures, as well as native P7 whole rat lung, were sequenced for comparison. We find that native-like AEC2 phenotype, alveolar structure, and cellular signaling patterns are better recapitulated via epithelial-mesenchymal-endothelial tri-culture rather than by a more simplified system. FBs cultured in the presence of endothelial cells demonstrate reduced expression of contractile markers and upregulate features of an AEC2 niche-supportive signature.
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| Overall design |
Single-cell RNA sequencing data of AEC2/fibroblast and AEC2/fibroblast/endothelial (tri-culture) d7 engineered lungs, with AEC2 and fibroblast isolates, primary ECs, as well as P7 native rat lung (pooled from 6 rats) for comparison
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| Contributor(s) |
Leiby KL, Niklason LE |
| Citation(s) |
37117221 |
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| Submission date |
Jun 17, 2021 |
| Last update date |
May 03, 2023 |
| Contact name |
Katherine L Leiby |
| E-mail(s) |
katherine.leiby@yale.edu
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| Organization name |
Yale University
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| Department |
Biomedical Engineering
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| Lab |
Niklason Lab
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| Street address |
10 Amistad St. Rm 314
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| City |
New Haven |
| State/province |
CT |
| ZIP/Postal code |
06519 |
| Country |
USA |
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| Platforms (2) |
| GPL22396 |
Illumina HiSeq 4000 (Rattus norvegicus) |
| GPL25947 |
Illumina NovaSeq 6000 (Rattus norvegicus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA738933 |
| SRA |
SRP324528 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE178405_RAW.tar |
471.9 Mb |
(http)(custom) |
TAR (of MTX, TSV, TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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