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| Status |
Public on Jun 18, 2021 |
| Title |
Expression data from SIRT6 knockout, young WT and old WT mice brains |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Brain-specific SIRT6-KO mice present increased DNA damage, learning impairments, and neurodegenerative phenotypes, placing SIRT6 as a key protein in preventing neurodegeneration. In the aging brain, SIRT6 levels/activity decline, which is accentuated in Alzheimer's patients. To understand SIRT6 roles in transcript pattern changes, we analyzed transcriptomes of young WT, old WT and young SIRT6-KO mice brains, and found changes in gene expression related to healthy and pathological aging. In addition, we traced these differences in human and mouse samples of Alzheimer's and Parkinson's diseases, healthy aging and calorie restriction (CR). Our results define four gene expression categories that change with age in a pathological or non-pathological manner, which are either reversed or not by CR. We found that each of these gene expression categories is associated with specific transcription factors, thus serving as potential candidates for their category-specific regulation. One of these candidates is YY1, which we found to act together with SIRT6 regulating specific processes. We thus argue that SIRT6 has a pivotal role in preventing age-related transcriptional changes in brains. Therefore, reduced SIRT6 activity may drive pathological age-related gene expression signatures in the brain.
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| Overall design |
Whole-exon microarray analysis of brain samples from either old, full-body SIRT6-KO or WT mice.
Overall 9 samples were analyzed: 3 Old WT (22-26 months old), 3 young WT/het (21 days old; no phenotypical difference between WT and heterozygous), 3 young SIRT6KO (21 days old).
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| Contributor(s) |
Toiber D |
| Citation(s) |
33690173 |
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| Submission date |
Jun 17, 2021 |
| Last update date |
Jul 17, 2021 |
| Contact name |
Debra Toiber |
| E-mail(s) |
toiber@bgu.ac.il, debbie.toiber@gmail.com
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| Phone |
0584449189
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| Organization name |
Ben Gurion University of the Negev
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| Street address |
Ben Gurion Road #1, Building 40, room 205
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| City |
Beer Sheva |
| State/province |
Israel |
| ZIP/Postal code |
8410501 |
| Country |
Israel |
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| Platforms (1) |
| GPL6193 |
[MoEx-1_0-st] Affymetrix Mouse Exon 1.0 ST Array [probe set (exon) version] |
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| Samples (9)
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| GSM5391022 |
Old WT mouse brain, biological replicate 1 |
| GSM5391023 |
Old WT mouse brain, biological replicate 2 |
| GSM5391024 |
Old WT mouse brain, biological replicate 3 |
| GSM5391025 |
Young WT mouse brain, 21 days old, biological replicate 1 |
| GSM5391026 |
Young WT mouse brain, 21 days old, biological replicate 2 |
| GSM5391027 |
Young SIRT6 heterozygous mouse brain, 21 days old, biological replicate 3 |
| GSM5391028 |
Young SIRT6KO mouse brain, 21 days old, biological replicate 1 |
| GSM5391029 |
Young SIRT6KO mouse brain, 21 days old, biological replicate 2 |
| GSM5391030 |
Young SIRT6KO mouse brain, 21 days old, biological replicate 3 |
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| Relations |
| BioProject |
PRJNA738953 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE178432_RAW.tar |
221.5 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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