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| Status |
Public on Jun 08, 2010 |
| Title |
Gene expression profile after CCM1 over expression in human umbilical vein endothelial cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
CCM1 (also known as KRIT1) is critical regulator of endothelial cell biology. Mutations in CCM1 can lead to the formation of cerebral cavernous malformations (CCM). CCM lesions are frequent in the human population; however, their pathogenesis is poorly understood. This genome-wide expression analysis is aimed to unravelling novel mechanisms how CCM1 executes its functions in endothelial cells. We used human umbilical vein endothelial cells (HUVEC) as a model system to study CCM1 functions after adenoviral over expression. The results of this experiment suggest that CCM1 is a critical regulator of endothelial cell cycle control and proliferation as well as migration and angiogenesis. This fits well to the roles of CCM1 in HUVEC which indeed acts as a negative regulator of angiogenesis.
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| Overall design |
Human umbilical vein endothelial cells were transduced with adenovirus expressing CCM1 or GFP as control. 48 hours after transduction total RNA was isolated from duplicates for genome-wide expression analysis
biological replicate: GFP rep1, GFP rep2
biological replicate: CCM1 rep1, CCM1 rep2
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| |
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| Contributor(s) |
Fischer A, Wuestehube J, Liebler SS, Bartol A |
| Citation(s) |
20616044 |
| |
| Submission date |
Sep 08, 2009 |
| Last update date |
Jan 18, 2013 |
| Contact name |
Andreas Fischer |
| URL |
http://www.angiolab.de
|
| Organization name |
German Cancer Research Center and Medical Faculty Mannheim, Heidelberg University
|
| Department |
Joint Research Division Vascular Biology
|
| Street address |
Ludolf-Krehl-Str. 13-17
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| City |
Mannheim |
| ZIP/Postal code |
68167 |
| Country |
Germany |
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| Platforms (1) |
| GPL6104 |
Illumina humanRef-8 v2.0 expression beadchip |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA119243 |
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