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| Status |
Public on Sep 01, 2021 |
| Title |
Transcriptome profiling of Candidatus Liberibacter asiaticus in citrus and psyllids |
| Organisms |
Citrus x paradisi; Diaphorina citri; Citrus macrophylla |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Candidatus Liberibacter asiaticus (Las) is an emergent bacterial pathogen associated with the devastating citrus Huanglongbing (HLB), also known as the greening disease. Vectored by the Asian Citrus Psyllid (Diaphorina citri), Las colonizes the phloem tissue of citrus. So far, efforts of cultivating Las in vitro have not been successful and dual-transcriptome analyses could only detect ~100 Las genes due to the low abundance of bacterial RNA in infected citrus/psyllid tissues. Therefore, the biology of this pathogen is poorly understood. Here, we established a procedure to enrich Las RNA for transcriptome analysis in order to obtain insights into the interactions of Las with its two hosts. We were able to confidently determine the expression profiles of >400 Las genes, including 106 that were differentially expressed between citrus and psyllids. Genes related to transcription/translation and defense were found to be upregulated in citrus; whereas genes upregulated in psyllids are involved in metabolic pathways related to tricarboxylic acid (TCA) cycle. Genes encoding the succinate dehydrogenase and NADH quinone oxidoreductase complexes, as well as the flagellar system are also expressed to higher levels in psyllids. We also analyzed the relative expression levels of Sec-delivered effectors, which are considered key virulence factors of Las. This work advances our understanding of the HLB biology and offers novel insight into the HLB pathogenesis.
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| Overall design |
Samples collected from midribs on HLB-infected citrus (grapefruit and macrophylla) and Asian Citrus Psyllid (ACP) insects. RNA samples underwent various pre-enrichment and post-enrichment treatments for CLas transcripts
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| Contributor(s) |
Ma W, Lovelace AH, De Francesco A |
| Citation(s) |
35025694 |
| |
| Submission date |
Jul 22, 2021 |
| Last update date |
Jan 14, 2022 |
| Contact name |
Wenbo Ma |
| Organization name |
The Sainsbury Laboratory
|
| Lab |
Ma Lab
|
| Street address |
Norwich Research Park
|
| City |
Norwich |
| ZIP/Postal code |
NR4 7UH |
| Country |
United Kingdom |
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| Platforms (3) |
| GPL30419 |
Illumina HiSeq 4000 (Citrus macrophylla) |
| GPL30420 |
Illumina HiSeq 4000 (Citrus x paradisi) |
| GPL30421 |
Illumina HiSeq 4000 (Diaphorina citri) |
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| Samples (22)
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| Relations |
| BioProject |
PRJNA748963 |
| SRA |
SRP329435 |
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