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| Status |
Public on Aug 06, 2021 |
| Title |
Next-generation sequencing facilitates quantitative analysis of rat BMSCs in soft or stiff SA hydrogel |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Purpose: To identify genes and the molecular pathways involved in the MSCs response to extracellular matrix stiffness, we performed RNA-sequencing of MSCs which cultured in soft (2 kPa) and stiff (18 kPa) SA hydrogels.
Methods: mRNA profiles of MSCs cultured in soft (2 kPa) and stiff (18 kPa) SA hydrogel for 48 h were generated by deep sequencing, in quadruplicate, using Illumina HiSeq 2000.
Results: Using an optimized data analysis workflow, we identified 33950 transcripts in MSCs with BWA workflow.
Conclusions: Our results present the detailed analysis of MSCs transcriptomes cultured in soft (2 kPa) and stiff (18 kPa) matrix, and found that matrix stiffness dominated multiple mRNA pathways in MSCs.
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| Overall design |
The mRNA profiles of MSCs in soft and stiff hydrogel were generated by deep sequencing, in quadruplicate, using Illumina HiSeq 2000.
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| Contributor(s) |
Cai K, Lin C |
| Citation missing |
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| |
| Submission date |
Aug 05, 2021 |
| Last update date |
Aug 07, 2021 |
| Contact name |
chuanchuan lin |
| E-mail(s) |
chuanchuanlin3@gmail.com
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| Phone |
+8613896191363
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| Organization name |
Chongqing University
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| Street address |
Shayang Road, Shapingba distract,
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| City |
Chongqing |
| State/province |
China |
| ZIP/Postal code |
400044 |
| Country |
China |
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| Platforms (1) |
| GPL14844 |
Illumina HiSeq 2000 (Rattus norvegicus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA752332 |
| SRA |
SRP331209 |
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