|
| Status |
Public on Feb 02, 2022 |
| Title |
IRF1 and IFNγ regulated gene expression in A549 lung epithelial cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Identification and comparison of genes induced by IRF1 ectopic expression versus IFNγ treatment in A549 lung epithelial fibroblasts.
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| |
|
| Overall design |
IRF1 or FLUC control were ectopically expressed in A549s using a lentiviral expression cassette. 72 h after transduction, FLUC expressing cells were further treated with or without 1000 U/mL IFNγ for 12 h. All samples were subsequently harvested for analysis. Three biological replicates were conducted per sample. For RNA-seq analysis, changes in gene expression with IRF1 ectopic expression or IFNγ treatment were determined via comparison to untreated cells ectopically expressing FLUC control.
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| |
|
| Contributor(s) |
Rinkenberger NR, Sibley D |
| Citation(s) |
34871166 |
| |
| Submission date |
Aug 11, 2021 |
| Last update date |
Feb 02, 2022 |
| Contact name |
Sibley David |
| Organization name |
Washington University in St. Louis
|
| Street address |
660 S. Euclid, CB 8230
|
| City |
St. Louis |
| State/province |
Missouri |
| ZIP/Postal code |
63110-0931 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (9)
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|
| Relations |
| BioProject |
PRJNA753768 |
| SRA |
SRP332051 |
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