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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Mar 01, 2010 |
| Title |
miR-29 targets in human fetal lung fibroblast IMR-90 cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
TGFβ is one of most intensively studied regulators of extracellular matrix formation, and has been implicated in the development of pulmonary fibrosis in different models. However, little is know about the role of miRNAs in TGFβ mediated fibrogenic gene regulation. By using miRNA qRT-PCR array, we have identified miRNAs whose expression are regulated by TGFβ in IMR-90 cells. Among those down-regulated miRNAs are miR-29 family members. Knockdown miR-29 in IMR-90 cells results in up-regulation of a large number of extracellular matrix and fibrogenic genes including family members of collagen, laminin, integrin, ADAM and MMP, many of them are predicted or confirmed miR-29 targets. Hierarchichal clustering analysis of mRNA array data revealed that many extracellular matrix and fibrogenic genes up-regulated by TGFβ in IMR-90 cells, are also up-regulated in miR-29 KD cells. Moreover, the similar set of extracellular matrix and fibrogenic genes is also significantly up-regulated in bleomycin treated mouse lungs. Together, our data strongly suggest that downstream of the TGFβ, miR-29 is a master modulator of genes involved in extracellular matrix formation and might play a significant role in pulmonary fibrosis.
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| Overall design |
To identify targets of miR-29 in human fetal lung fibroblast cells (IMR-90), cells were transfected with 100nM scrambled, 50nM or 100nM of miR-29 LNA KD oligo. Total RNA were isolated 48hrs after transfection and subject to the mRNA array analysis using Affymetrix human Gene 1.0 ST Whole Genome Array
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| Contributor(s) |
Lu J, Cushing L, Kuang P |
| Citation(s) |
20971881 |
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| Submission date |
Oct 20, 2009 |
| Last update date |
Jul 26, 2018 |
| Contact name |
jining lu |
| E-mail(s) |
jining@bu.edu
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| Phone |
617-638-6180
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| Fax |
617-536-8093
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| Organization name |
Boston University
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| Street address |
715 Albany street R304
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| City |
BoSTON |
| ZIP/Postal code |
02118 |
| Country |
USA |
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| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA121587 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE18651_RAW.tar |
35.4 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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