|
| Status |
Public on Sep 15, 2022 |
| Title |
Time-regulated transcripts with the potential to modulate human pluripotent stem cell-derived cardiomyocyte differentiation [miRNA] |
| Platform organism |
synthetic construct |
| Sample organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
Human pluripotent stem cell-derived cardiomyocytes (hPSC-CM) are a promising disease model, although hPSC-CMs are not fully mature cardiomyocytes. We examined exponential differentially expressed miRNA/gene to identify critical time-regulated transcripts associated with hPSC-CM differentiation. We uncovered 324 interactions among 29 differentially expressed genes and 51 miRNAs from 20.543 transcripts during the 120 days of hPSC-CM differentiation. We found 16 genes and 26 miRNAs with an inverse pattern of expression (Pearson R-values < -0.5) validated using several human and mouse databases. We further validated these findings using two hPSC-CM lines and seven sampling times over a 30-day protocol and observed 16 inverse interactions among eight genes and 12 miRNAs (Person R-values < -0.5) changing over time. Finally, we tested the top eight miRNAs by adding miRNA mimics to differentiating hPSC-CMs and found that they influenced proliferation and maturation phenotypes. These time-regulated transcripts appear to regulate single or multiple pathways affecting cardiac differentiation.
|
| |
|
| Overall design |
microRNA expression array of human pluripotent stem cells (hPSCs), hPSCs progenitor cardiomyocytes, and hiPSC early cardiomyocytes at different time-points of differentiation were analyzed in duplicates at 0, 2, 4, 6, 8, 15 and 30 days of maturation
microRNA extracted from hPSC commited to cardiomyocytes differentiation protocol in different time points of development
|
| |
|
| Contributor(s) |
Munõz JJ, Dariolli R, Silva CM, Neri EA, Turaça LT, Carvalho ML, Velho MR, Sobie EA, Krieger JE |
| Citation(s) |
36056380 |
| |
| Submission date |
Nov 12, 2021 |
| Last update date |
Sep 16, 2022 |
| Contact name |
Jose Eduardo Krieger |
| E-mail(s) |
krieger@incor.usp.br
|
| Phone |
+551126615573
|
| Organization name |
University of Sao Paulo
|
| Department |
Medical School
|
| Lab |
Laboratory of Genetics and Molecular Cardiology (LGCM)
|
| Street address |
44 Av. Dr. Enéas de Carvalho Aguiar
|
| City |
Sao Paulo |
| State/province |
Sao Paulo |
| ZIP/Postal code |
05403-000 |
| Country |
Brazil |
| |
|
| Platforms (1) |
| GPL19117 |
[miRNA-4] Affymetrix Multispecies miRNA-4 Array |
|
| Samples (14)
|
| GSM5688965 |
hPSCs at Day 0 of differentiation (D0_A05)_miRNA |
| GSM5688966 |
hPSC progenitor cardiomyocytes at Day 2 of differentiation (D2_B05)_miRNA |
| GSM5688967 |
hPSC progenitor cardiomyocytes at Day 4 of differentiation (D4_C05)_miRNA |
| GSM5688968 |
hPSC early cardiomyocytes at Day 6 of differentiation (D6_D05)_miRNA |
| GSM5688969 |
hPSC early cardiomyocytes at Day 8 of differentiation (D8_E05)_miRNA |
| GSM5688970 |
hPSC cardiomyocytes at Day 15 of differentiation (D15_F05)_miRNA |
| GSM5688971 |
hPSC cardiomyocytes at Day 30 of differentiation (D30_G05)_miRNA |
| GSM5688972 |
hPSCs at Day 0 of differentiation (D0_A07)_miRNA |
| GSM5688973 |
hPSC progenitor cardiomyocytes at Day 2 of differentiation (D2_B07)_miRNA |
| GSM5688974 |
hPSC progenitor cardiomyocytes at Day 4 of differentiation (D4_C07)_miRNA |
| GSM5688975 |
hPSC early cardiomyocytes at Day 6 of differentiation (D6_D07)_miRNA |
| GSM5688976 |
hPSC early cardiomyocytes at Day 8 of differentiation (D8_E07)_miRNA |
| GSM5688977 |
hPSC cardiomyocytes at Day 15 of differentiation (D15_F07)_miRNA |
| GSM5688978 |
hPSC cardiomyocytes at Day 30 of differentiation (D30_G07)_miRNA |
|
| This SubSeries is part of SuperSeries: |
| GSE188749 |
Time-regulated transcripts with the potential to modulate human pluripotent stem cell-derived cardiomyocyte differentiation |
|
| Relations |
| BioProject |
PRJNA780000 |
Less...
More...