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| Status |
Public on Feb 27, 2023 |
| Title |
ribo-seq in control and U1 AMO treated HeLa cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
Other
|
| Summary |
Control and U1 AMO were transfected into Hela cells, Ribosome-associated RNAs were purified and sequenced in Novaseq platform.To compare the translation efficiency, mRNA-seq were performed in parallel.
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| Overall design |
To understand the ribosome-binding profile of mRNAs in control and U1 AMO treated HeLa cells, we carried out Ribo-seq analysis in control and U1 AMO treated HeLa cells. To compare the translation efficiency, we performed mRNA-seq analysis in parallel.
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| |
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| Contributor(s) |
Deng Y, Ran Y, Feng Q, Yao C |
| Citation(s) |
37224962 |
| |
| Submission date |
Jan 07, 2022 |
| Last update date |
May 29, 2023 |
| Contact name |
chengguo yao |
| E-mail(s) |
yaochguo@mail.sysu.edu.cn
|
| Organization name |
sysu
|
| Department |
school of medicine
|
| Street address |
No. 74 zhongshan 2rd road
|
| City |
guangzhou |
| ZIP/Postal code |
510080 |
| Country |
China |
| |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (8)
|
| GSM5776309 |
U1 mRNA-seq |
| GSM6347756 |
mRNAseq analysis in hela cells treated with control amo |
| GSM6347757 |
mRNAseq analysis in hela cells treated with u1 amo |
| GSM6347758 |
riboseq analysis in hela cells treated with control amo |
| GSM6347759 |
riboseq analysis in hela cells treated with u1 amo |
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| This SubSeries is part of SuperSeries: |
| GSE192943 |
The U1 antisense morpholino oligonucleotide (AMO) disrupts U1 snRNP structure to promote intronic PCPA modification of pre-mRNAs |
|
| Relations |
| BioProject |
PRJNA795419 |
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