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| Status |
Public on Dec 03, 2010 |
| Title |
Gene expression changes in mouse forebrain deficient in Dnmt1 and Dnmt3a |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Two major DNA methylation-catalyzing enzymes, Dnmt1 and Dnmt3a, are expressed in postmitotic neurons, but their function in the adult central nervous system is unclear. We generated conditional mutant mice (CamKIIa Cre; Dnmt loxP) that lack either Dnmt1 or Dnmt3a, or both, exclusively in forebrain excitatory neurons and found only double-knockout (DKO) mice exhibited abnormal hippocampal CA1 long-term plasticity and deficits of learning and memory. DKO neurons also exhibit a significant up-regulation of immune genes, such as class I MHC and Stat1, which are implicated in synaptic plasticity.
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| Overall design |
Four pairs of 2-3 month-old DKO and litter mate control were used. RNA samples were extracted from the cortex and hippocampus for gene expression array analysis.
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| Contributor(s) |
Feng J, Fan G |
| Citation(s) |
20729844, 20228804 |
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| Submission date |
Dec 07, 2009 |
| Last update date |
Mar 09, 2020 |
| Contact name |
jian feng |
| Organization name |
UCLA
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| Department |
Human Genetics
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| Lab |
Guoping Fan
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| Street address |
695 Charles E Young Dr
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| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90095 |
| Country |
USA |
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| Platforms (1) |
| GPL2872 |
Agilent-012694 Whole Mouse Genome G4122A (Feature Number version) |
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| Samples (4)
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| GSM480802 |
Adult brain, Dnmt1 Dnmt3a double knockout (DKO), replicate 1 |
| GSM480803 |
Adult brain, Dnmt1 Dnmt3a double knockout (DKO), replicate 2 |
| GSM480804 |
Adult brain, Dnmt1 Dnmt3a double knockout (DKO), replicate 3 |
| GSM480805 |
Adult brain, Dnmt1 Dnmt3a double knockout (DKO), replicate 4 |
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| Relations |
| BioProject |
PRJNA120407 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE19367_RAW.tar |
51.5 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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