|
| Status |
Public on Mar 03, 2022 |
| Title |
RNA-sequencing of bicyclomycin treated Escherichia coli str. O157:H7 str. Sakai |
| Organism |
Escherichia coli |
| Experiment type |
Expression profiling by high throughput sequencing
Other
|
| Summary |
Enterohaemorrhagic E. coli (EHEC) is a significant human pathogens that cause outbreaks of haemorrhagic colitis and haemolytic uremic syndrome. During infection, pathogens compete for iron with the host, and one mechanism by which EHEC obtains iron is through haem uptake and utilitisation which is encoded by the chu operon. We have demonstrated that the haem receptor chuA is regulated by the Crp-cAMP-dependent sRNA CyaR. We further demonstrate that activation of chuA by CyaR is independent of the chuA RNA-thermometer and termination by Rho. These results highlight the ability of regulatory sRNAs to integrate multiple environmental signals into a layered hierarchy of signal input.
|
| |
|
| Overall design |
Detecting rho-dependent transcripts in Escherichia coli str. O157:H7 str. Sakai
|
| |
|
| Contributor(s) |
Sy BM, Tree JJ |
| Citation(s) |
35422774 |
| |
| Submission date |
Feb 24, 2022 |
| Last update date |
Apr 20, 2022 |
| Contact name |
Jai Justin Tree |
| E-mail(s) |
j.tree@unsw.edu.au
|
| Phone |
+61 2 938 59142
|
| Organization name |
University of New South Wales
|
| Department |
School of Biotechnology and Biomolecular Sciences
|
| Lab |
Tree lab
|
| Street address |
Rm s110 Bldg F25, UNSW, Gate 11 Botany St
|
| City |
Sydney |
| State/province |
NSW |
| ZIP/Postal code |
2033 |
| Country |
Australia |
| |
|
| Platforms (1) |
| GPL21222 |
Illumina NextSeq 500 (Escherichia coli) |
|
| Samples (6)
|
| GSM5915327 |
bicyclomycin treated Escherichia coli str. O157:H7 str. Sakai- replicate 1 |
| GSM5915328 |
bicyclomycin treated Escherichia coli str. O157:H7 str. Sakai- replicate 2 |
| GSM5915329 |
bicyclomycin treated Escherichia coli str. O157:H7 str. Sakai- replicate 3 |
| GSM5915330 |
untreated Escherichia coli str. O157:H7 str. Sakai- replicate 1 |
| GSM5915331 |
untreated Escherichia coli str. O157:H7 str. Sakai- replicate 2 |
| GSM5915332 |
untreated Escherichia coli str. O157:H7 str. Sakai- replicate 3 |
|
| Relations |
| BioProject |
PRJNA810054 |
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